Overview

  • Product nameAnti-Galectin 3 antibody [A3A12]
    See all Galectin 3 primary antibodies
  • Description
    Mouse monoclonal [A3A12] to Galectin 3
  • SpecificityBy Western blot, this antibody detects an ~30 kDa protein representing Galectin 3 from Jurkat cells transfected with human Galectin 3. Immunohistochemical staining of Galectin 3 in rat olfactory bulb yields a pattern consistent with nuclear and plasma membrane staining.
  • Tested applicationsSuitable for: ELISA, IHC-Fr, WB, ICC/IF, Inhibition Assay, IHC-P, ICCmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Galectin 3.

  • EpitopeThe epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.
  • Positive control
    • WB: Jurkat cells transfected with human galectin-3 IHC: rat olfactory bulb.
  • General notes

     

    This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesThis antibody has been shown to potentiate the binding of Galectin 3 to IgG.
  • ClonalityMonoclonal
  • Clone numberA3A12
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use a concentration of 6 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ICC/IF 1/20 - 1/200.
Inhibition Assay Use at an assay dependent concentration.
IHC-P 1/20 - 1/200.
ICC 1/200.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
    • Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
    • Sequence similaritiesContains 1 galectin domain.
    • Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • 35 kDa lectin antibody
      • Carbohydrate binding protein 35 antibody
      • Carbohydrate-binding protein 35 antibody
      • CBP 35 antibody
      • CBP35 antibody
      • Gal-3 antibody
      • GAL3 antibody
      • Galactose-specific lectin 3 antibody
      • Galactoside-binding protein antibody
      • GALBP antibody
      • Galectin 3 internal gene,included antibody
      • Galectin-3 antibody
      • Galectin3 antibody
      • GALIG antibody
      • GBP antibody
      • IgE binding protein antibody
      • IgE-binding protein antibody
      • L 31 antibody
      • L 34 antibody
      • L-31 antibody
      • L-34 galactoside-binding lectin antibody
      • L31 antibody
      • Laminin-binding protein antibody
      • Lectin L-29 antibody
      • Lectin, galactose binding, soluble 3 antibody
      • LEG3_HUMAN antibody
      • LGALS2 antibody
      • LGALS3 antibody
      • MAC 2 antigen antibody
      • Mac-2 antibody
      • Mac-2 antigen antibody
      • MAC2 antibody
      • Macrophage galactose-specific lectin antibody
      • MGC105387 antibody
      see all

    Anti-Galectin 3 antibody [A3A12] images

    • ab2785 labelling Galectin 3 (green) in the cytoplasm and nucleus of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • ab2785 labelling Galectin 3 (green) in the cytoplasm and nucleus of NIH-3T3 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • ab2785 labelling Galectin 3 (green) in the cytoplasm and nucleus of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • ab2785 labelling Galectin 3 in the nucleus and cytoplasm of Human ovarian carcinoma tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • ab2785 labelling Galectin 3 in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • ab2785 labelling Galectin 3 in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH-3T3 cell lysate

      Lysates/proteins at 25 µg/ml per lane.


      Predicted band size : 26 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)


    • Predicted band size : 26 kDa
      Western blot using ab2785 at 1:1000. Review by Bo Su submitted 26 July 2004.
      Western blot using ab2785 at 1:1000. Review by Bo Su submitted 26 July 2004
    • ICC/IF image of ab2785 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2785, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab2785 staining Galectin 3 in rat spleen tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in paraformaldehyde, blocked with 20% serum for 20 minutes at 24°C, then incubated with ab2785 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-mouse polyclonal used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

      See Abreview

    References for Anti-Galectin 3 antibody [A3A12] (ab2785)

    This product has been referenced in:
    • Lin YW  et al. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages. J Mol Cell Cardiol 79:287-94 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25528964) »
    • Wu J  et al. Cell cycle activation contributes to increased neuronal activity in the posterior thalamic nucleus and associated chronic hyperesthesia after rat spinal cord contusion. Neurotherapeutics 10:520-38 (2013). WB ; Rat . Read more (PubMed: 23775067) »

    See all 11 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (Tumors slides)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization No
    Specification Tumors slides
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Mr. Thiago Vargas

    Verified customer

    Submitted Apr 05 2016

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample Human Tissue sections (Retina)
    Specification Retina
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 17 2014

    Thank you for contacting us.

    We use a PBS pH 7,4 +/- 0.1 containing Potassium as well as Sodium phosphate, KCL and NaCl.

    For a liter of a 20x stock we use: Potassium phosphate monobasic 4,8 gNaCl 160 gKCL 4 gSodium phosphate dibasic 2...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (Kelly Neuroblastoma cell line)
    Specification Kelly Neuroblastoma cell line
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Rat Cell (33B Cell line)
    Specification 33B Cell line
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (33B Neuroblastoma cell line)
    Specification 33B Neuroblastoma cell line
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Kelly Neuroblastoma cell line)
    Specification Kelly Neuroblastoma cell line
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    Thank you for contacting us.

    We recommend a starting concentration of 0.1ug/ml when using purified antibodies in ELISA. This will have to optimized for your specific protocol.

    Should you need a positive control for your ELISA we...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Spleen)
    Specification Spleen
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 30 2011

    Thank you very much for your valuable feedback. I think it is possible that these higher bands are dimers and trimers, given the size they are migrating at. If the bond is particularly strong, it may be that the complex is not dissociated by reduc...

    Read More

    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"