Overview

  • Product nameAnti-Galectin 3 antibody
    See all Galectin 3 primary antibodies
  • Description
    Rabbit polyclonal to Galectin 3
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region from the internal domain of Human Galectin 3 (NP_002297.2).

  • Positive control
    • HeLa cell extract; Human Colon (epithelium) and Spleen (macrophages); NIH 3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: PBS without Mg2+, Ca2+
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similaritiesContains 1 galectin domain.
  • Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

Anti-Galectin 3 antibody images

  • All lanes : Anti-Galectin 3 antibody (ab118851) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLA cell extract with synthesized peptide


    Predicted band size : 26 kDa
  • ab118851, at 10 µg/ml, staining Galectin 3 in Formalin-fixed, Paraffin-embedded Human Colon, eipithelium tissue by Immunohistochemistry.
  • ab118851, at 10 µg/ml, staining Galectin 3 in Formalin-fixed, Paraffin-embedded Human Spleen (macrophages) tissue by Immunohistochemistry.
  • ab118851 at 1/100 dilution staining Galectin 3 in NIH 3T3 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

References for Anti-Galectin 3 antibody (ab118851)

This product has been referenced in:
  • Zhang H  et al. Galectin-3 as a marker and potential therapeutic target in breast cancer. PLoS One 9:e103482 (2014). IHC-P ; Human . Read more (PubMed: 25254965) »

See 1 Publication for this product

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