Overview

  • Product name
  • Description
    Rabbit polyclonal to Galectin 3
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Galectin 3.

    (Peptide available as ab31706.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell and Nuclear Lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

Target

  • Function
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificity
    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similarities
    Contains 1 galectin domain.
  • Cellular localization
    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

Images



  • Predicted band size : 26 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)              
    Lane 2: Galectin 3  knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab31707 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab31707 was shown to specifically recognize Galectin 3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Galectin 3 knockout samples were examined. Wild-type and Galectin 3 knockout samples were subjected to SDS-PAGE.  Ab31707 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ICC/IF image of ab31707 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31707, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-Galectin 3 antibody (ab31707) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    We also see a very faint band at 52 kDa in the HeLa Whole cell lysate, we are uncertain as to the identity of this band.

References

This product has been referenced in:
  • Truchan HK  et al. Type II Secretion Substrates of Legionella pneumophila Translocate Out of the Pathogen-Occupied Vacuole via a Semipermeable Membrane. MBio 8:N/A (2017). ICC/IF . Read more (PubMed: 28634242) »
  • Coppin L  et al. Galectin-3 is a non-classic RNA binding protein that stabilizes the mucin MUC4 mRNA in the cytoplasm of cancer cells. Sci Rep 7:43927 (2017). Read more (PubMed: 28262838) »

See all 6 Publications for this product

Customer reviews and Q&As

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Please find the SDS document for the anti-Galectin 3 antibody (ab31707) attached to this email. This can now also be accessed from the datasheet of ab31707.

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Thank you for contacting us yesterday in regards to the anti-Galectin 3 antibody (ab31707).

As discussed over the phone I have checked the sequence homology of the human protein (from which the immunogen of this antibody was taken) to that of...

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DISCOUNT CODE: *** Expiration date: April 14th, 2012 I am very pleased to hear you would like to accept our offer and test ab31707 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast carcinoma)
Loading amount
50 µg
Specification
Breast carcinoma
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 18 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (HT1080)
Specification
HT1080
Fixative
Paraformaldehyde
Permeabilization
Yes - 4%
Username

Abcam user community

Verified customer

Submitted May 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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