Validated using a knockout cell line

Anti-Galectin 3 antibody [EPR2774] (ab76466)


  • Product name
    Anti-Galectin 3 antibody [EPR2774]
    See all Galectin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR2774] to Galectin 3
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Galectin 3

  • Positive control
    • WB: HeLa, SW480, A431 and MCF7 cell lysates ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/30 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
    • Tissue specificity
      A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
    • Sequence similarities
      Contains 1 galectin domain.
    • Cellular localization
      Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • 35 kDa lectin antibody
      • Carbohydrate binding protein 35 antibody
      • Carbohydrate-binding protein 35 antibody
      • CBP 35 antibody
      • CBP35 antibody
      • Gal-3 antibody
      • GAL3 antibody
      • Galactose-specific lectin 3 antibody
      • Galactoside-binding protein antibody
      • GALBP antibody
      • Galectin 3 internal gene,included antibody
      • Galectin-3 antibody
      • Galectin3 antibody
      • GALIG antibody
      • GBP antibody
      • IgE binding protein antibody
      • IgE-binding protein antibody
      • L 31 antibody
      • L 34 antibody
      • L-31 antibody
      • L-34 galactoside-binding lectin antibody
      • L31 antibody
      • Laminin-binding protein antibody
      • Lectin L-29 antibody
      • Lectin, galactose binding, soluble 3 antibody
      • LEG3_HUMAN antibody
      • LGALS2 antibody
      • LGALS3 antibody
      • MAC 2 antigen antibody
      • Mac-2 antibody
      • Mac-2 antigen antibody
      • MAC2 antibody
      • Macrophage galactose-specific lectin antibody
      • MGC105387 antibody
      see all

    Anti-Galectin 3 antibody [EPR2774] images

    • Predicted band size : 26 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Galectin 3 (KO) knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76466 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab76466 was shown to specifically react with Galectin 3 (KO) when Galectin 3 (KO) knockout samples were used. Wild-type and Galectin 3 (KO) knockout samples were subjected to SDS-PAGE. Ab76466 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing THP1 cells stained with ab76466 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76466, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab76466, at a 1/100 dilution, staining Galectin 3 in HeLa cells by Immunofluorescence.
    • All lanes : Anti-Galectin 3 antibody [EPR2774] (ab76466) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 26 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References for Anti-Galectin 3 antibody [EPR2774] (ab76466)

    ab76466 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Western blot
    Mouse Cell lysate - whole cell (BMDM)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris gel, MES running buffer)
    Loading amount
    5 µg
    Blocking step
    Licor TBS blocking buffer as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 22°C

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    Verified customer

    Submitted Aug 24 2016


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