Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
Contains 1 galectin domain.
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: Galectin 3 knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: MCF7 whole cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab86058 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab86058 was shown to recognize Galectin 3 when Galectin 3 (KO) knockout samples were used, along with additional cross-reactive bands. Wild-type and Galectin 3 knockout samples were subjected to SDS-PAGE. ab86058 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Galectin 3 antibody [RM0082-5L36] (ab86058)