Recombinant
RabMAb

Anti-Galectin 7 antibody [EPR4287] (ab108623)

Overview

  • Product name
    Anti-Galectin 7 antibody [EPR4287]
    See all Galectin 7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4287] to Galectin 7
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Galectin 7. The exact sequence is proprietary.

  • Positive control
    • WB: Human skin tissue lysate and HACAT cell lysate. IHC-P: Human skin tissue. Flow Cyt: HACAT cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Galectin 7 antibody (Alexa Fluor® 647) [EPR4287] (ab207047)

    Anti-Galectin 7 antibody (Alexa Fluor® 488) [EPR4287] (ab207365)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/2000.

Target

  • Function
    Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
  • Tissue specificity
    Mainly expressed in stratified squamous epithelium.
  • Sequence similarities
    Contains 1 galectin domain.
  • Cellular localization
    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gal-7 antibody
    • GAL7 antibody
    • Galectin 7B antibody
    • Galectin-7 antibody
    • HKL-14 antibody
    • Human keratinocyte lectin 14 antibody
    • Keratinocyte lectin 14 antibody
    • Lectin galactoside binding soluble 7 antibody
    • Lectin, galactoside binding, soluble, 7B antibody
    • LEG7_HUMAN antibody
    • LGALS7 antibody
    • LGALS7A antibody
    • LGALS7B antibody
    • P53 induced protein 1 antibody
    • p53-induced gene 1 protein antibody
    • Pi7 antibody
    • PIG1 antibody
    • TP53I1 antibody
    see all

Anti-Galectin 7 antibody [EPR4287] images

  • All lanes : Anti-Galectin 7 antibody [EPR4287] (ab108623) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : HACAT cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 15 kDa
  • ab108623, at 1/500 dilution, staining Galectin 7 in paraffin-embedded Human skin tissue by Immunohistochemistry.
  • Flow cytometry analysis of HACAT (Human skin keratinocyte) cells labeling Galectin 7 (red) with ab108623 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • ab108623 showing negative staining in Normal liver tissue.

  • ab108623 showing negative staining in Normal liver tissue.

  • ab108623 showing negative staining in Normal kidney tissue.

  • ab108623 showing negative staining in Normal heart tissue.

  • ab108623 showing negative staining in Normal brain tissue.

References for Anti-Galectin 7 antibody [EPR4287] (ab108623)

This product has been referenced in:
  • Guo JP & Li XG Galectin-7 promotes the invasiveness of human oral squamous cell carcinoma cells via activation of ERK and JNK signaling. Oncol Lett 13:1919-1924 (2017). WB ; Human . Read more (PubMed: 28454344) »
  • Jiang Y  et al. Clinical significance of galectin-7 in vulvar squamous cell carcinoma. Oncol Lett 10:3826-3831 (2015). WB . Read more (PubMed: 26788216) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab108623.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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