The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
1/10 - 1/100.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt.
FunctionCould be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
Tissue specificityMainly expressed in stratified squamous epithelium.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.