Overview

  • Product nameAnti-Galectin 8 antibody
    See all Galectin 8 primary antibodies
  • Description
    Rabbit polyclonal to Galectin 8
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Human, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human galectin 8.

    (Peptide available as ab42878.)

  • Positive control
    • This antibody gave a positive signal in Galectin 8 Tagged Recombinant Protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab42879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa corresponding to the molecular weight of the Galectin 8 tagged recombinant protein (predicted molecular weight of Galectin 8: 35 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Galectin 8 antibody images

    • Anti-Galectin 8 antibody (ab42879) at 1 µg/ml + Galectin 8 Recombinant Protein at 0.1 µg

      Secondary
      Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 35 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    References for Anti-Galectin 8 antibody (ab42879)

    ab42879 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab42879.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"