Overview

  • Product name
  • Description
    Rabbit polyclonal to Galectin 8
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized peptide derived from an internal region of human Galectin 8.

  • Positive control
    • Extracts from 3T3 cells and human prostate carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab69631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects bands of approximately 36 and 60 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity
      Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
    • Sequence similarities
      Contains 2 galectin domains.
    • Domain
      Contains two homologous but distinct carbohydrate-binding domains.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Gal 8 antibody
      • Gal-8 antibody
      • Gal8 antibody
      • Galectin-8 antibody
      • galectin-8g antibody
      • Lectin galactoside binding soluble 8 antibody
      • LEG8_HUMAN antibody
      • LGAL S8 antibody
      • Lgals8 antibody
      • PCTA 1 antibody
      • PCTA-1 antibody
      • PCTA1 antibody
      • Po66 carbohydrate binding protein antibody
      • Po66 carbohydrate-binding protein antibody
      • Po66 CBP antibody
      • Po66-CBP antibody
      • Prostate carcinoma tumor antigen 1 antibody
      see all

    Images

    • All lanes : Anti-Galectin 8 antibody (ab69631) at 1/500 dilution

      Lane 1 : extracts of 3T3 cells
      Lane 2 : extracts of 3T3 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using ab69631 at 1:50 dilution.
      Left image un-treated.
      Right image treated with immunizing peptide.

    References

    This product has been referenced in:
    • Reticker-Flynn NE & Bhatia SN Aberrant glycosylation promotes lung cancer metastasis through adhesion to galectins in the metastatic niche. Cancer Discov 5:168-81 (2015). Read more (PubMed: 25421439) »
    • Reticker-Flynn NE  et al. A combinatorial extracellular matrix platform identifies cell-extracellular matrix interactions that correlate with metastasis. Nat Commun 3:1122 (2012). WB, IHC ; Mouse . Read more (PubMed: 23047680) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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