Anti-galectin 9/Gal-9 antibody (ab228686)
Key features and details
- Rabbit polyclonal to galectin 9/Gal-9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-galectin 9/Gal-9 antibody
See all galectin 9/Gal-9 primary antibodies -
Description
Rabbit polyclonal to galectin 9/Gal-9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human galectin 9/Gal-9 (internal sequence). The exact sequence is proprietary.
Database link: O00182 -
Positive control
- WB: Galectin 9/Gal-9-transfected HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
Target
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Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
Tissue specificity
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. -
Cellular localization
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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Database links
- Entrez Gene: 3965 Human
- Omim: 601879 Human
- SwissProt: O00182 Human
- Unigene: 81337 Human
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Alternative names
- 36 kDa beta-galactoside-binding lectin antibody
- Ecalectin antibody
- Gal-9 antibody
see all
Images
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All lanes : Anti-galectin 9/Gal-9 antibody (ab228686) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Galectin 9/Gal-9-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228686 has not yet been referenced specifically in any publications.