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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab69630? Please let us know so that we can cite the reference in this datasheet
ab69630 has been referenced in 1 publications.
- Alam S et al. Galectin-9 expression in endothelial cells is positively regulated by histone deacetylase 3. J Biol Chem : (2011). WB, ICC/IF; Human. PubMed: 22027828
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - galectin 9 antibody (ab69630)
All lanes : Anti-galectin 9 antibody (ab69630) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 40 kDa
Observed band size : 40 kDa
Immunocytochemistry/ Immunofluorescence - galectin 9 antibody (ab69630)
Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-galectin 9 antibody(ab69630)
ab69360 (4µg/ml) staining Galectin 9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Anti-galectin 9 antibody (ab69630)
Anti-galectin 9 antibody (ab69630) at 1/500 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
Ab69630 recognizes the partial tagged recombinant Human galectin 9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
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