• Product nameAnti-galectin 9 antibody
    See all galectin 9 primary antibodies
  • Description
    Rabbit polyclonal to galectin 9
  • SpecificityThis antibody detects endogenous levels of total Galectin-9 protein.
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human galectin 9 aa 51-100 (internal sequence).
    Database link: O00182

  • Positive control
    • NIH/3T3 cells. Extracts from HepG2 cells, human lymphoma



Our Abpromise guarantee covers the use of ab69630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.


  • FunctionBinds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • Tissue specificityPeripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • Sequence similaritiesContains 2 galectin domains.
  • DomainContains two homologous but distinct carbohydrate-binding domains.
  • Cellular localizationCytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ecalectin antibody
    • Gal-9 antibody
    • Galectin-9 antibody
    • galectin9 antibody
    • HOM HD 21 antibody
    • HOMHD21 antibody
    • HUAT antibody
    • Lectin galactoside binding soluble 9 antibody
    • LEG9_HUMAN antibody
    • LGAL S9 antibody
    • LGALS 9 antibody
    • Lgals9 antibody
    • LGALS9A antibody
    • MGC117375 antibody
    • MGC125973 antibody
    • MGC125974 antibody
    • Tumor antigen HOM-HD-21 antibody
    • Urate transporter/channel protein antibody
    see all

Anti-galectin 9 antibody images

  • All lanes : Anti-galectin 9 antibody (ab69630) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • ab69360 (4µg/ml) staining Galectin 9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.

References for Anti-galectin 9 antibody (ab69630)

This product has been referenced in:
  • Jiang J  et al. Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer. PLoS One 8:e81799 (2013). IHC-P ; Human . Read more (PubMed: 24339967) »
  • Alam S  et al. Galectin-9 expression in endothelial cells is positively regulated by histone deacetylase 3. J Biol Chem : (2011). WB, ICC/IF ; Human . Read more (PubMed: 22027828) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neurons)
Specification Neurons
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Paraformaldehyde

Gokhan Yilmaz

Verified customer

Submitted Sep 21 2016

Thank you for contacting us.

I am confirming that I have logged your phone call in our system. If you have any future inquiry please do not hesitate to call us back again.

I hope this information is helpful to you. Please do not hesit...

Read More

Thank you for your reply.

Please let me know if you have any success by changing the blocking buffer. It is actually not that uncommon to load such amounts of whole cell lysates, however if that is not feasible for your experiments I am...

Read More

Thank you for contacting Abcam regarding ab69630.

I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions to impro...

Read More

Thank you for your reply.

We did not test this antibody with a standard protein.This antibody can be used for ELISA due to validation by the following:

1) Peptide-Based ELISA: Coat immunogen peptide in 96-well plate. Det...

Read More

Thank you once again for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As requested, I have obtained a copy of the testing protocol. Please bear in mind that this...

Read More

Thank you for contacting Abcam regarding ab69630.

Regarding our testing conditions for ELISA, this was tested for immunogen-coated ELISA using a conjugated secondary antibody. It has not been tested in sandwich ELISA.


Read More

Thank you for your enquiry. I have contacted the originator of the product, but unfortunately, there is only limited amount of the peptide and is not available for purchase. I am sorry I can't be of more assistance in this occasion but if there...

Read More