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Products:Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Overview
Product name
Anti-galectin 9 antibody
See all galectin 9 products (3) ...
Description
Rabbit polyclonal to galectin 9
Tested applications
IHC-P, WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from the internal region of human galectin 9.
Positive control
NIH/3T3 cells. Extracts from HepG2 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell differentiation
Neuroscience >> Cell Adhesion Proteins >> Nuclear Proteins
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/500 - 1/1000.Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
ICC/IF: 1/500 - 1/1000.
Target
Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
Tissue specificity
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
Sequence similarities
Contains 2 galectin domains.
Domain
Contains two homologous but distinct carbohydrate-binding domains.
Cellular localization
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
Target information above from: UniProt accessionO00182
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ecalectin antibody
- Gal-9 antibody
- Galectin-9 antibody
see all
Database links
- Entrez Gene: 3965 Human
- Entrez Gene: 16859 Mouse
- Entrez Gene: 25476 Rat
- Omim: 601879 Human
- SwissProt: O00182 Human
- SwissProt: Q3B8N1 Human
- SwissProt: O08573 Mouse
- SwissProt: P97840 Rat
see all
Anti-galectin 9 antibody images:
Western blot - galectin 9 antibody (ab69630)
All lanes : Anti-galectin 9 antibody (ab69630) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 40 kDa
Observed band size : 40 kDa
Immunocytochemistry/ Immunofluorescence - galectin 9 antibody (ab69630)
Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-galectin 9 antibody(ab69630)
ab69360 (4µg/ml) staining Galectin 9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-galectin 9 antibody (ab69630)
This product has been referenced in:
- Alam Set al. Galectin-9 expression in endothelial cells is positively regulated by histone deacetylase 3. J Biol Chem : (2011). WB, ICC/IF; Human.Read more (PubMed: 22027828) »
See 1 publication for this product
Publishing research using ab69630? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"