• Product name
  • Description
    Rabbit polyclonal to GALK1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) from the N terminus of GALK1.

  • Positive control
    • extracts from HUVEC cells and HeLa cells.



Our Abpromise guarantee covers the use of ab65123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.


  • Function
    Major enzyme for galactose metabolism.
  • Pathway
    Carbohydrate metabolism; galactose metabolism.
  • Involvement in disease
    Defects in GALK1 are the cause of galactosemia II (GALCT2) [MIM:230200]. Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses.
  • Sequence similarities
    Belongs to the GHMP kinase family. GalK subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC antibody
    • epididymis secretory protein Li 19 antibody
    • Galactokinase 1 antibody
    • Galactokinase antibody
    • Galactose kinase antibody
    • GALK 1 antibody
    • GALK antibody
    • GALK1 antibody
    • GALK1_HUMAN antibody
    • GK1 antibody
    • GLK antibody
    • GLK1 antibody
    • HEL-S-19 antibody
    see all

Anti-GALK1 antibody images

  • All lanes : Anti-GALK1 antibody (ab65123) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HeLa cells
    Lane 3 : extracts from HeLa cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GALK1 antibody (ab65123)

ab65123 has not yet been referenced specifically in any publications.

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