Overview

  • Product nameAnti-GALNT1 antibody
    See all GALNT1 primary antibodies
  • Description
    Mouse polyclonal to GALNT1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human GALNT1 protein (AAH47746.1)

  • Positive control
    • GALNT1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of <10 kDa (predicted molecular weight: Isoform 2, 12 kDa; Isoform 1, 64 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.
    • Tissue specificityWidely expressed. Expressed in all tissues tested.
    • PathwayProtein modification; protein glycosylation.
    • Sequence similaritiesBelongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
      Contains 1 ricin B-type lectin domain.
    • DomainThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
      The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
    • Cellular localizationSecreted and Golgi apparatus > Golgi stack membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GALNAC T1 antibody
      • GalNAc transferase 1 antibody
      • GalNAc-T1 antibody
      • GALNT 1 antibody
      • GALNT1 antibody
      • GALT1_HUMAN antibody
      • Polypeptide GalNAc transferase 1 antibody
      • Polypeptide N acetylgalactosaminyltransferase 1 antibody
      • Polypeptide N-acetylgalactosaminyltransferase 1 soluble form antibody
      • pp GaNTase 1 antibody
      • pp-GaNTase 1 antibody
      • Protein UDP acetylgalactosaminyltransferase 1 antibody
      • Protein-UDP acetylgalactosaminyltransferase 1 antibody
      • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1 antibody
      • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 (GalNAc T1) antibody
      • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 antibody
      • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 antibody
      see all

    Anti-GALNT1 antibody images

    • All lanes : Anti-GALNT1 antibody (ab69959) at 1/500 dilution

      Lane 1 : GALNT1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 12 kDa
      Observed band size : <10 kDa (why is the actual band size different from the predicted?)

    References for Anti-GALNT1 antibody (ab69959)

    ab69959 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab69959.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"