Overview

  • Product name
  • Description
    Rabbit polyclonal to GALNT10
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 16 amino acids near the N terminal of Human GALNT10 (NP_938080).

  • Positive control
    • Rat brain tissue lysate; Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab106445 was purified by affinity chromatography via a peptide column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward Muc5Ac and EA2 peptide substrates.
  • Tissue specificity
    Widely expressed. Expressed at high level in small intestine, and at intermediate levels in stomach, pancreas, ovary, thyroid gland and spleen. Weakly expressed in other tissues.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • Domain
    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586H0623 antibody
    • FLJ00205 antibody
    • FLJ11715 antibody
    • GalNAc T10 antibody
    • GalNAc transferase 10 antibody
    • GalNAc-T10 antibody
    • GalNAcT10 antibody
    • GALNT10 antibody
    • GLT10_HUMAN antibody
    • Polypeptide GalNAc transferase 10 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 10 antibody
    • pp GalNAc T10 antibody
    • pp GaNTase 10 antibody
    • pp-GaNTase 10 antibody
    • ppGalNAcT10 antibody
    • ppGaNTase 10 antibody
    • Protein UDP acetylgalactosaminyltransferase 10 antibody
    • Protein-UDP acetylgalactosaminyltransferase 10 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 10 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 10 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10 antibody
    • UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10) antibody
    see all

Images

  • Immunofluorescence of GALNT10 in Human brain tissue with ab106445 at 20 ug/mL.

  • Immunohistochemistry of GALNT10 in Human brain tissue with ab106455 at 2.5 μg/mL.

  • Lane 1 : Anti-GALNT10 antibody (ab106445) at 1 µg/ml
    Lane 2 : Anti-GALNT10 antibody (ab106445) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 69 kDa

References

ab106445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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