- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-GALNT10 antibody
See all GALNT10 primary antibodies -
DescriptionRabbit polyclonal to GALNT10
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
An 18 amino acid synthetic peptide near the C terminus of Human GALNT10 (NP_938080).
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Positive control
- SK-N-SH cell lysate; Human brain tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab106471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 69 kDa. | |
| IHC-P | Use a concentration of 2.5 µg/ml. |
Target
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FunctionCatalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward Muc5Ac and EA2 peptide substrates.
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Tissue specificityWidely expressed. Expressed at high level in small intestine, and at intermediate levels in stomach, pancreas, ovary, thyroid gland and spleen. Weakly expressed in other tissues.
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PathwayProtein modification; protein glycosylation.
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Sequence similaritiesBelongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
DomainThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. -
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
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Database links
- Entrez Gene: 55568 Human
- Entrez Gene: 171212 Mouse
- Entrez Gene: 170501 Rat
- Omim: 608043 Human
- SwissProt: Q86SR1 Human
- SwissProt: Q6P9S7 Mouse
- SwissProt: Q925R7 Rat
- Unigene: 631797 Human
see all -
Alternative names
- DKFZp586H0623 antibody
- FLJ00205 antibody
- FLJ11715 antibody
see all
Anti-GALNT10 antibody images
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Western blot - GALNT10 antibody (ab106471)Lane 1 : Anti-GALNT10 antibody (ab106471) at 1 µg/ml
Lane 2 : Anti-GALNT10 antibody (ab106471) at 2 µg/ml
Lane 1 : SK-N-SH cell lysate
Lane 2 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GALNT10 antibody (ab106471)ab106471, at 2.5 µg/ml, staining GALNT10 in Human brain tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence-Anti-GALNT10 antibody(ab106471)Immunofluorescence of GALNT10 in Human Brain cells using ab106471 at 20 ug/ml.
Protocols
References for Anti-GALNT10 antibody (ab106471)
ab106471 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"