Overview

  • Product name
  • Description
    Rabbit polyclonal to GALNT12
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 34-173 of Human GALNT12 (AAH13945; UniProt ID: Q8IXK2 isoform 2).

  • Positive control
    • A549 whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: for isoform 2, 32 kDa.

Target

  • Function
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.
  • Tissue specificity
    Widely expressed at different levels of expression. Highly expressed in digestive organs such as small intestine, stomach, pancreas and colon. Expressed at intermediate level in testis, thyroid gland and spleen. Weakly expressed in whole brain, cerebral cortex, cerebellum, fetal brain, bone marrow, thymus, leukocytes, heart, skeletal muscle, liver, lung, esophagus, kidney, adrenal gland, mammary gland, uterus, placenta, ovary and prostate.
  • Pathway
    Protein modification; protein glycosylation.
  • Involvement in disease
    Defects in GALNT12 are a cause of susceptibility to colorectal cancer type 1 (CRCS1) [MIM:608812]. Colorectal cancer is a malignancy originating either in the colon or rectum or both.
  • Sequence similarities
    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • Domain
    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Colorectal cancer susceptibility to 1 antibody
    • CRCS1 antibody
    • FLJ21212 antibody
    • GalNAc T12 antibody
    • GalNAc-T12 antibody
    • GalNAcT12 antibody
    • GALNT 12 antibody
    • Galnt12 antibody
    • GLT12_HUMAN antibody
    • Polypeptide GalNAc transferase 12 antibody
    • Polypeptide N acetylgalactosaminyltransferase 12 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 12 antibody
    • pp GaNTase 12 antibody
    • pp-GaNTase 12 antibody
    • Protein UDP acetylgalactosaminyltransferase 12 antibody
    • Protein-UDP acetylgalactosaminyltransferase 12 antibody
    • UDP GalNAc polypeptide N acetylgalactosaminyltransferase 12 antibody
    • UDP GalNAc polypeptide N acetylgalactosaminyltransferase antibody
    • UDP N acetyl alpha D galactosamine polypeptide N acetylgalactosaminyltransferase 12 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 12 antibody
    see all

Anti-GALNT12 antibody images

  • Anti-GALNT12 antibody (ab101358) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : for isoform 2, 32 kDa

References for Anti-GALNT12 antibody (ab101358)

ab101358 has not yet been referenced specifically in any publications.

Product Wall

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