Overview

  • Product name
  • Description
    Rabbit polyclonal to GALNT13
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 38-87 (KERSLLPALR AVISRNQEGP GEMGKAVLIP KDDQEKMKEL FKINQFNLMA) of Human GALNT13 (NP_443149).

  • Positive control
    • Hela cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab81422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/1562500.

Target

  • Relevance
    GALNT13 is a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAcT; EC 2.4.1.41) family, which initiate O-linked glycosylation of mucins by the initial transfer of N-acetylgalactosamine (GalNAc) with an alpha-linkage to a serine or threonine residue. It is specifically expressed in neuronal cells; expressed in fetal brain, whole adult brain, cerebral cortex and cerebellum; not expressed in other tissues tested. There are two named isoforms.
  • Cellular localization
    Golgi apparatus membrane; Single pass type II membrane protein.
  • Database links
  • Alternative names
    • GalNAc T13 antibody
    • GalNAc transferase 13 antibody
    • H_NH0187G20.1 antibody
    • N acetylgalactosaminyltransferase 13 antibody
    • Polypeptide GalNAc transferase 13 antibody
    • Polypeptide N acetylgalactosaminyltransferase 13 antibody
    • pp GaNTase 13 antibody
    • Protein UDP acetylgalactosaminyltransferase 13 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 13 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 13 (GalNAc T13) antibody
    • WUGSC:H_NH0187G20.1 antibody
    see all

Images

  • Anti-GALNT13 antibody (ab81422) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit Ig at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

References

ab81422 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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