Overview

  • Product name
    Anti-GALNT3 antibody
  • Description
    Rabbit polyclonal to GALNT3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 93-142 (PERPCLQGYY TAAELKPVLD PPQDSNAPGA GKPFKITHLS EEQKEKE) of Rat GALNT3 (NP_001015032).

  • Positive control
    • Rat brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Glycosylates FGF23. Plays a central role in phosphate homeostasis.
  • Tissue specificity
    Expressed in organs that contain secretory epithelial glands. Highly expressed in pancreas, skin, kidney and testis. Weakly expressed in prostate, ovary, intestine and colon. Also expressed in placenta and lung and fetal lung and fetal kidney.
  • Pathway
    Protein modification; protein glycosylation.
  • Involvement in disease
    Defects in GALNT3 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • Sequence similarities
    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • Domain
    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane. Resides preferentially in the trans and medial parts of the Golgi stack.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C10199 antibody
    • EC 2.4.1.41 antibody
    • GalNAc T3 antibody
    • GalNAc transferase 3 antibody
    • GalNAc-T3 antibody
    • GalNAcT3 antibody
    • GALNT3 antibody
    • GALT3_HUMAN antibody
    • HFTC antibody
    • HHS antibody
    • MGC61909 antibody
    • OTTHUMP00000204915 antibody
    • OTTHUMP00000204919 antibody
    • Polypeptide GalNAc transferase 3 antibody
    • Polypeptide GalNAc transferase T3 antibody
    • Polypeptide N acetylgalactosaminyltransferase 3 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 3 antibody
    • pp-GaNTase 3 antibody
    • ppGaNTase 3 antibody
    • Protein UDP acetylgalactosaminyltransferase 3 antibody
    • Protein-UDP acetylgalactosaminyltransferase 3 antibody
    • RP23-306G16.2 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 3 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 3 (GalNAc T3) antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 antibody
    • UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 antibody
    see all

Anti-GALNT3 antibody images

  • Anti-GALNT3 antibody (ab113880) at 1 µg/ml + Rat brain lysate at 10 µg

    Predicted band size : 73 kDa

References for Anti-GALNT3 antibody (ab113880)

ab113880 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (lysate)
Specification
lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Oct 27 2014

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (colon and breast cancer)
Loading amount
50 µg
Specification
colon and breast cancer
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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