Overview

  • Product name
  • Description
    Mouse polyclonal to GALNT4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Full length protein, corresponding to amino acids 1-578 of Human GALNT4 (AAH36390.1).

  • Positive control
    • GALNT4 transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 67 kDa.

Target

  • Function
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. Glycosylates 'Thr-57' of SELPLG.
  • Tissue specificity
    Ubiquitous. Highly expressed in mucous cells.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • Domain
    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.4.1.41 antibody
    • GALNAC T4 antibody
    • GalNAc transferase 4 antibody
    • GalNAc-T4 antibody
    • GalNAcT4 antibody
    • GALNT4 antibody
    • GALT4_HUMAN antibody
    • N-acetylgalactosaminyltransferase 4 (GalNAc-T4) antibody
    • Polypeptide GalNAc transferase 4 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 4 antibody
    • pp GaNTase 4 antibody
    • pp-GaNTase 4 antibody
    • Protein UDP acetylgalactosaminyltransferase 4 antibody
    • Protein-UDP acetylgalactosaminyltransferase 4 antibody
    • UDP GalNAc:polypeptide N-acetylgalactosaminyltransferase 4 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4 antibody
    • UDP-N-acetyl-alpha-D-galactosamine:polypeptide antibody
    see all

Images

  • All lanes : Anti-GALNT4 antibody (ab167648) at 1/500 dilution

    Lane 1 : GALNT4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Goat Anti-Mouse IgG HRP antibody at 1/2500 dilution

    Predicted band size : 67 kDa

References

ab167648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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