Overview

  • Product name
    Anti-GALNT8 antibody
  • Description
    Rabbit polyclonal to GALNT8
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 502-629 of Human GALNT8 (Q9NY28).

  • Positive control
    • Human small intestine tissues; RT4, U251 MG, Human liver and Human tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Probably catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.
  • Tissue specificity
    Widely expressed. Expressed in heart, skeletal muscle, kidney, liver, small intestine and placenta. Weakly expressed in colon, thymus, spleen, lung and leukocyte.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • Domain
    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GALNAC T8 antibody
    • GalNAc-T8 antibody
    • GALNT8 antibody
    • GALT8_HUMAN antibody
    • Polypeptide GalNAc transferase 8 antibody
    • pp-GaNTase 8 antibody
    • Probable polypeptide N-acetylgalactosaminyltransferase 8 antibody
    • Protein-UDP acetylgalactosaminyltransferase 8 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 8 antibody
    see all

Images

  • Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles. Antibody staining is shown in green.

  • ab121374 at 1/75 dilution staining GALNT8 in Paraffin-embedded Human Small intestine tissue by Immunohistochemistry.
  • All lanes : Anti-GALNT8 antibody (ab121374) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab121374 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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