Validated using a knockout cell line
Recombinant
RabMAb

Anti-GALT antibody [EPR12555] (ab178406)

Overview

  • Product name
    Anti-GALT antibody [EPR12555]
    See all GALT primary antibodies
  • Description
    Rabbit monoclonal [EPR12555] to GALT
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GALT aa 50-150. The exact sequence is proprietary.
    Database link: P07902

  • Positive control
    • K562, MDA-MB-435 and HepG2 cell lysates; Human fetal brain and fetal liver lysates; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab178406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 43 kDa.
IP 1/10 - 1/100.

Target

  • Pathway
    Carbohydrate metabolism; galactose metabolism.
  • Involvement in disease
    Defects in GALT are the cause of galactosemia (GALCT) [MIM:230400]. Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation.
  • Sequence similarities
    Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gal 1 P uridylyltransferase antibody
    • Gal-1-P uridylyltransferase antibody
    • Galactose 1 phosphate uridyl transferase antibody
    • Galactose 1 phosphate uridylyltransferase antibody
    • Galactose-1-phosphate uridylyltransferase antibody
    • GALT antibody
    • GALT_HUMAN antibody
    • UDP glucose hexose 1 phosphate uridylyltransferase antibody
    • UDP-glucose--hexose-1-phosphate uridylyltransferase antibody
    see all

Images



  • Predicted band size : 43 kDa

    Lanes 1, 3 and 5: GALT knockout HAP1 cell lysate (20 µg)

    Lanes 2, 4 and 6: Wild-type HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Green signal from target – ab178406 observed at 43 kDa

    Lanes 3 and 4: Red signal from loading control – ab8245 observed at 37 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab178406 was shown to react with GALT when GALT knockout samples were used, along with additional cross-reactive bands. Wild-type and GALT knockout samples were subjected to SDS-PAGE. ab178406 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : MDA-MB-435 cell lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human fetal liver lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 43 kDa
  • Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing GALT, immunoprecipitated using ab178406 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

ab178406 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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