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Anti-gamma Adaptin antibody [100/3] (ab11328)

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Overview

Product name

Anti-gamma Adaptin antibody [100/3]
See all gamma Adaptin products (5) ...

Description

Mouse monoclonal [100/3] to gamma Adaptin

Specificity

The antibody reacts with polypeptides of approximately 100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources, or from rat pheochromocytoma cultured cells (PC12), neuroblastoma or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody does not recognize the gamma-subunit in rat and mouse. Using immunofluorescent staining on MDBK cells, and African green monkey kidney cells the binding of the antibody appears to be largely confined to the trans-Golgi network.

Tested applications

ICC/IF, WB, IP, Electron Microscopymore details

Cross reactivity

Reacts with

Cow, Human

Does not react with

Mouse, Rat

Immunogen

Full length protein (Cow).

General notes

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

100/3

Isotype

IgG2b

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ICC/IF

     

  • WB

     

  • IP

     

  • Electron Microscopy

     

Application notes

Electron Microscopy: 1/100.
ICC/IF: 1/500(from PMID 17404221).
IP: 1/100.
WB: 1/100. Detects a band of approximately 104 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes large subunit family.
Contains 1 GAE domain.

Cellular localization

Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Target information above from: UniProt accessionO43747 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Adapter-related protein complex 1 subunit gamma-1 antibody
  • Adaptor protein complex AP 1 gamma 1 subunit antibody
  • Adaptor protein complex AP 1 subunit gamma 1 antibody
  • Adaptor protein complex AP-1 subunit gamma-1 antibody
  • Adaptor related protein complex 1 gamma 1 subunit antibody
  • adaptor-related protein complex 1 gamma 1 subunit antibody
  • ADTG antibody
  • AP 1 complex subunit gamma 1 antibody
  • AP-1 complex subunit gamma-1 antibody
  • AP1G1 antibody
  • AP1G1_HUMAN antibody
  • CLAPG1 antibody
  • Clathrin assembly protein complex 1 gamma 1 large chain antibody
  • Clathrin assembly protein complex 1 gamma large chain antibody
  • Clathrin assembly protein complex 1 gamma-1 large chain antibody
  • Clathrin associated/assembly/adaptor protein large gamma 1 antibody
  • clathrin-associated/assembly/adaptor protein large gamma 1 antibody
  • Gamma1 adaptin antibody
  • Gamma1-adaptin antibody
  • Golgi adaptor HA1/AP1 adaptin gamma subunit antibody
  • Golgi adaptor HA1/AP1 adaptin subunit gamma 1 antibody
  • Golgi adaptor HA1/AP1 adaptin subunit gamma-1 antibody
  • MGC18255 antibody
see all

Database links

References for Anti-gamma Adaptin antibody [100/3] (ab11328)

This product has been referenced in:

  • Duncan MCet al. Composite synthetic lethal identification of membrane traffic inhibitors. Proc Natl Acad Sci U S A 104:6235-40 (2007).Read more (PubMed: 17404221) »
  • Swigut Tet al. Cooperative interactions of simian immunodeficiency virus Nef, AP-2, and CD3-zeta mediate the selective induction of T-cell receptor-CD3 endocytosis. J Virol 77:8116-26 (2003).Read more (PubMed: 12829850) »

See all 6 publications for this product

Publishing research using ab11328? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"