gamma Adaptin antibody [100/3] (ab11328)

Product Name 

gamma Adaptin antibody [100/3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [100/3] to gamma Adaptin

Immunogen 

Full length protein (Cow).

Reacts with 

Hu, Cow

Does not react with 
(species key)

Ms, Rat

Specificity 

The antibody reacts with polypeptides of approximately 100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources, or from rat pheochromocytoma cultured cells (PC12), neuroblastoma or astrocytes.
This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain.
The antibody does not recognize the gamma-subunit in rat and mouse.
Using immunofluorescent staining on MDBK cells, and African green monkey kidney cells the binding of the antibody appears to be largely confined to the trans-Golgi network.

Tested applications 
(see key)

EM, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Electron Microscopy: 1/100.
ICC/IF: 1/500(from PMID 17404221).
IP: 1/100.
WB: 1/100. Detects a band of approximately 104 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters

Relevance 

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta, beta prime and gamma adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

100/3

Isotype 

IgG2b

Purity 

Ascites

Storage buffer 

Preservative: 0.1% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.400 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

References for gamma Adaptin antibody [100/3] (ab11328)

  This product has been used in: (two most recent references)

Duncan MC et al. Composite synthetic lethal identification of membrane traffic inhibitors. Proc Natl Acad Sci U S A 104:6235-40 (2007). PubMed: 17404221

Swigut T et al. Cooperative interactions of simian immunodeficiency virus Nef, AP-2, and CD3-zeta mediate the selective induction of T-cell receptor-CD3 endocytosis. J Virol 77:8116-26 (2003). PubMed: 12829850

See all 6 publication references for this product.


If you publish research using ab11328 please let us know so that we can cite the reference on this datasheet.

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