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All lanes : Anti-gamma Adaptin antibody (ab21980) at 1/2000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 91 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
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