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Anti-gamma Adaptin antibody (ab21980)

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Overview

Product name

Anti-gamma Adaptin antibody
See all gamma Adaptin products (5) ...

Description

Rabbit polyclonal to gamma Adaptin

Tested applications

IP, WB, IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide: IRLRELIRTIRTARTQAEER, corresponding to N terminal amino acids 4-23 of gamma Adaptin

IRLRELIRTI RTARTQAEER

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Rat brain lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum

Purity

Whole antiserum

Purification notes

0.1ml filtered lyophilized serum

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters

  • Western blot - gamma Adaptin antibody (ab21980)Western blot - gamma Adaptin antibody (ab21980) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab21980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-Fr: 1/200 - 1/2000. depending on tissue and detection method.
IHC-P: 1/200 - 1/2000. depending on tissue and detection method.
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/4000. depending on detection method. Predicted molecular weight: 91 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes large subunit family.
Contains 1 GAE domain.

Cellular localization

Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Target information above from: UniProt accessionO43747 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Adapter-related protein complex 1 subunit gamma-1 antibody
  • Adaptor protein complex AP 1 gamma 1 subunit antibody
  • Adaptor protein complex AP 1 subunit gamma 1 antibody
  • Adaptor protein complex AP-1 subunit gamma-1 antibody
  • Adaptor related protein complex 1 gamma 1 subunit antibody
  • adaptor-related protein complex 1 gamma 1 subunit antibody
  • ADTG antibody
  • ADTG antibody
  • AP 1 complex subunit gamma 1 antibody
  • AP-1 complex subunit gamma-1 antibody
  • AP1G1 antibody
  • AP1G1_HUMAN antibody
  • CLAPG1 antibody
  • CLAPG1 antibody
  • Clathrin assembly protein complex 1 gamma 1 large chain antibody
  • Clathrin assembly protein complex 1 gamma large chain antibody
  • Clathrin assembly protein complex 1 gamma-1 large chain antibody
  • Clathrin associated/assembly/adaptor protein large gamma 1 antibody
  • clathrin-associated/assembly/adaptor protein large gamma 1 antibody
  • Gamma1 adaptin antibody
  • Gamma1-adaptin antibody
  • Golgi adaptor HA1/AP1 adaptin gamma subunit antibody
  • Golgi adaptor HA1/AP1 adaptin subunit gamma 1 antibody
  • Golgi adaptor HA1/AP1 adaptin subunit gamma-1 antibody
  • MGC18255 antibody
see all

Database links

see all

Anti-gamma Adaptin antibody images:

  Western blot - gamma Adaptin antibody (ab21980)

Western blot - gamma Adaptin antibody (ab21980)

All lanes : Anti-gamma Adaptin antibody (ab21980) at 1/2000 dilution

Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size : 91 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References for Anti-gamma Adaptin antibody (ab21980)

This product has been referenced in:

  • Yi Let al. HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking. J Biol Chem 285:30884-905 (2010).Read more (PubMed: 20622010) »

See 1 publication for this product

Publishing research using ab21980? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"