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Western blot - gamma Adaptin antibody (ab21980)
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Product Name
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gamma Adaptin antibody
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See all gamma Adaptin antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to gamma Adaptin
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Immunogen
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Synthetic peptide: IRLRELIRTIRTARTQAEER, corresponding to N terminal amino acids 4-23 of gamma Adaptin
BLAST 'IRLRELIRTIRTARTQAEER' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: 1/200 - 1/2000. depending on tissue and detection method.
IHC-P: 1/200 - 1/2000. depending on tissue and detection method.
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/4000. depending on detection method. Predicted molecular weight: 91 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat brain lysate
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Cellular localization
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Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
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Relevance
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Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta, beta prime and gamma adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Purification notes
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0.1ml filtered lyophilized serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this gamma Adaptin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for gamma Adaptin antibody images, references, products related to ab21980 and other tools.
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gamma Adaptin antibody images:
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 Western blot - gamma Adaptin antibody (ab21980)
All lanes : gamma Adaptin antibody (ab21980) at 1/2000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 91 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to gamma Adaptin
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gamma Adaptin antibody - more information
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Customer reviews (feedback) regarding gamma Adaptin antibody |
Customer FAQs regarding gamma Adaptin antibody |
Protocols for gamma Adaptin antibody |
Price and availability of products related to gamma Adaptin antibody |
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gamma Adaptin antibody - related products:
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gamma Adaptin antibody - other tools:
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Contact Abcam with a Technical Enquiry about gamma Adaptin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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