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Anti-gamma Adaptin antibody (ab3706)

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Overview

Product name

Anti-gamma Adaptin antibody
See all gamma Adaptin products (5) ...

Description

Goat polyclonal to gamma Adaptin

Specificity

Recognises a band representing AP1G1 at just below 97kD that is blocked by the immunising peptide in HeLa extract.

Tested applications

WB, Electron Microscopymore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide: CTAPTSKPSSAGGEL, corresponding to amino acids 646 - 659 of Human AP1G1.

CTAPTSKPSS AGGEL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome

  • Western blot - gamma Adaptin antibody (ab3706)Western blot - gamma Adaptin antibody (ab3706) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Electron Microscopy: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 94 kDa (predicted molecular weight: 91 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes large subunit family.
Contains 1 GAE domain.

Cellular localization

Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Target information above from: UniProt accessionO43747 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Adapter-related protein complex 1 subunit gamma-1 antibody
  • Adaptor protein complex AP 1 gamma 1 subunit antibody
  • Adaptor protein complex AP 1 subunit gamma 1 antibody
  • Adaptor protein complex AP-1 subunit gamma-1 antibody
  • Adaptor related protein complex 1 gamma 1 subunit antibody
  • adaptor-related protein complex 1 gamma 1 subunit antibody
  • ADTG antibody
  • AP 1 complex subunit gamma 1 antibody
  • AP-1 complex subunit gamma-1 antibody
  • AP1G1 antibody
  • AP1G1_HUMAN antibody
  • CLAPG1 antibody
  • Clathrin assembly protein complex 1 gamma 1 large chain antibody
  • Clathrin assembly protein complex 1 gamma large chain antibody
  • Clathrin assembly protein complex 1 gamma-1 large chain antibody
  • Clathrin associated/assembly/adaptor protein large gamma 1 antibody
  • clathrin-associated/assembly/adaptor protein large gamma 1 antibody
  • Gamma1 adaptin antibody
  • Gamma1-adaptin antibody
  • Golgi adaptor HA1/AP1 adaptin gamma subunit antibody
  • Golgi adaptor HA1/AP1 adaptin subunit gamma 1 antibody
  • Golgi adaptor HA1/AP1 adaptin subunit gamma-1 antibody
  • MGC18255 antibody
see all

Database links

see all

Anti-gamma Adaptin antibody images:

  Western blot - gamma Adaptin antibody (ab3706)

Western blot - gamma Adaptin antibody (ab3706)



Predicted band size : 91 kDa


Western blot using ab3706 at 1/500.

Lane 1: HeLa Whole Cell Lysate
Lane 2: HeLa Whole Cell Lysate + 1µg/ml blocking/immunising peptide

Secondary ab: Rabbit polyclonal to Goat IgG HRP ab6741 (1/5000).
Exposure time: 1 minute.
Lysates at 20µg/lane.

Predicted molecular weight: 91 kDa. The strong band just under 97kD represents AP1G1.

References for Anti-gamma Adaptin antibody (ab3706)

This product has been referenced in:

  • Giles DKet al. Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion. Microbes Infect 8:1579-91 (2006).Read more (PubMed: 16698305) »

See 1 publication for this product

Publishing research using ab3706? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"