The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesElectron Microscopy: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 94 kDa (predicted molecular weight: 91 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the adaptor complexes large subunit family. Contains 1 GAE domain.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.