Anti-gamma Adaptin antibody (ab3706)

Overview

• Product nameAnti-gamma Adaptin antibodySee all gamma Adaptin primary antibodies ...
• Description
  Goat polyclonal to gamma Adaptin
• SpecificityRecognises a band representing AP1G1 at just below 97kD that is blocked by the immunising peptide in HeLa extract.
• Tested applicationsWB, Electron Microscopy more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide: CTAPTSKPSSAGGEL, corresponding to amino acids 646 - 659 of Human AP1G1.

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Vesicle Transport
  • Adapters

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Endosome

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Golgi

Applications

Target

• FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
• Tissue specificityWidely expressed.
• Sequence similaritiesBelongs to the adaptor complexes large subunit family.
  Contains 1 GAE domain.
• Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Target information above from: UniProt accession O43747 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 164 Human
  • Entrez Gene: 11765 Mouse
  • Entrez Gene: 171494 Rat
  • Omim: 603533 Human
  • SwissProt: O43747 Human
  • SwissProt: P22892 Mouse
  • Unigene: 461253 Human
  • Unigene: 37210 Mouse

  • Unigene: 430782 Mouse
  • Unigene: 471663 Mouse
  • Unigene: 9446 Rat

  see all

• Alternative names
  Adapter-related protein complex 1 subunit gamma-1 antibodyAdaptor protein complex AP 1 gamma 1 subunit antibodyAdaptor protein complex AP 1 subunit gamma 1 antibody

  Adaptor protein complex AP-1 subunit gamma-1 antibodyAdaptor related protein complex 1 gamma 1 subunit antibodyadaptor-related protein complex 1 gamma 1 subunit antibodyADTG antibodyAP 1 complex subunit gamma 1 antibodyAP-1 complex subunit gamma-1 antibodyAP1G1 antibodyAP1G1_HUMAN antibodyCLAPG1 antibodyClathrin assembly protein complex 1 gamma 1 large chain antibodyClathrin assembly protein complex 1 gamma large chain antibodyClathrin assembly protein complex 1 gamma-1 large chain antibodyClathrin associated/assembly/adaptor protein large gamma 1 antibodyclathrin-associated/assembly/adaptor protein large gamma 1 antibodyGamma1 adaptin antibodyGamma1-adaptin antibodyGolgi adaptor HA1/AP1 adaptin gamma subunit antibodyGolgi adaptor HA1/AP1 adaptin subunit gamma 1 antibodyGolgi adaptor HA1/AP1 adaptin subunit gamma-1 antibodyMGC18255 antibody

  see all

Anti-gamma Adaptin antibody images

• 

  Western blot - gamma Adaptin antibody (ab3706)
  
  
  Predicted band size : 91 kDa
  
  
  Western blot using ab3706 at 1/500.
  
  Lane 1: HeLa Whole Cell Lysate
  Lane 2: HeLa Whole Cell Lysate + 1µg/ml blocking/immunising peptide
  
  Secondary ab: Rabbit polyclonal to Goat IgG HRP ab6741 (1/5000).
  Exposure time: 1 minute.
  Lysates at 20µg/lane.
  
  Predicted molecular weight: 91 kDa. The strong band just under 97kD represents AP1G1.