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Anti-gamma Adaptin antibody
See all gamma Adaptin products (5) ...
Goat polyclonal to gamma Adaptin
Recognises a band representing AP1G1 at just below 97kD that is blocked by the immunising peptide in HeLa extract.
WB, Electron Microscopymore details
Reacts with
Human
Predicted to work with
Mouse, Rat
Synthetic peptide: CTAPTSKPSSAGGEL, corresponding to amino acids 646 - 659 of Human AP1G1.
CTAPTSKPSS AGGEL
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Western blot - gamma Adaptin antibody (ab3706)
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Our Abpromise guarantee covers the use of ab3706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 94 kDa (predicted molecular weight: 91 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Widely expressed.
Belongs to the adaptor complexes large subunit family.
Contains 1 GAE domain.
Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Target information above from: UniProt accessionO43747
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - gamma Adaptin antibody (ab3706)

Predicted band size : 91 kDa
Western blot using ab3706 at 1/500.
Lane 1: HeLa Whole Cell Lysate
Lane 2: HeLa Whole Cell Lysate + 1µg/ml blocking/immunising peptide
Secondary ab: Rabbit polyclonal to Goat IgG HRP ab6741 (1/5000).
Exposure time: 1 minute.
Lysates at 20µg/lane.
Predicted molecular weight: 91 kDa. The strong band just under 97kD represents AP1G1.
This product has been referenced in:
See 1 publication for this product
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Predicted band size : 91 kDa
Western blot using ab3706 at 1/500.
Lane 1: HeLa Whole Cell Lysate
Lane 2: HeLa Whole Cell Lysate + 1µg/ml blocking/immunising peptide
Secondary ab: Rabbit polyclonal to Goat IgG HRP ab6741 (1/5000).
Exposure time: 1 minute.
Lysates at 20µg/lane.
Predicted molecular weight: 91 kDa. The strong band just under 97kD represents AP1G1.
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