gamma Adaptin antibody (ab3706)

Product Name 

gamma Adaptin antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to gamma Adaptin

Immunogen 

Synthetic peptide: CTAPTSKPSSAGGEL, corresponding to amino acids 646 - 659 of Human AP1G1.
BLAST 'CTAPTSKPSSAGGEL' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

Recognises a band representing AP1G1 at just below 97kD that is blocked by the immunising peptide in HeLa extract.

Tested applications 
(see key)

EM, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Electron Microscopy: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 94 kDa (predicted molecular weight: 91 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome

Relevance 

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta, beta prime and gamma adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.700 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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gamma Adaptin antibody images:

References for gamma Adaptin antibody (ab3706)

  This product has been used in:

Giles DK et al. Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion. Microbes Infect 8:1579-91 (2006). PubMed: 16698305

If you publish research using ab3706 please let us know so that we can cite the reference on this datasheet.

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