Anti-gamma Actin antibody [2A3] (ab123034)

Overview

  • Product nameAnti-gamma Actin antibody [2A3]
    See all gamma Actin primary antibodies
  • Description
    Mouse monoclonal [2A3] to gamma Actin
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide (Ac-

    EEEIAALVI

    -COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin

  • Positive control
    • Human dermal fibroblast cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2A3
  • MyelomaNS0
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

Anti-gamma Actin antibody [2A3] images

  • Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml

  • ab123034 at 1/500 dilution staining gamma Actin in methanol-fixed Human dermal fibroblasts by Immunofluorescence.

References for Anti-gamma Actin antibody [2A3] (ab123034)

ab123034 has not yet been referenced specifically in any publications.

Product Wall

Yes , this antibody does not react with other isoforms and it binds to actin gamma 2 only.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"