Overview

  • Product nameAnti-gamma Actin antibody
    See all gamma Actin primary antibodies
  • Description
    Rabbit polyclonal to gamma Actin
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human gamma Actin. A peptide corresponding to residues surrounding amino acid 365 of Human gamma Actin.
    Database link: NM_001614

  • Positive control
    • WB: Human SW-620 cell lysates. IHC-P: Human fetal muscle tissue.

Properties

  • FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/100 - 1/500.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

Anti-gamma Actin antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal muscle tissue labelling gamma Actin with ab84479 at a dilution of 1/100.

  • Anti-gamma Actin antibody (ab84479) at 1/300 dilution + Human SW-620 cell lysates

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References for Anti-gamma Actin antibody (ab84479)

ab84479 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"