The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-Fr: 1/200 - 1/2000. depending on tissue and detection method.
IHC-P: 1/200 - 1/2000. depending on tissue and detection method.
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/4000. depending on detection method. Predicted molecular weight: 91 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the adaptor complexes large subunit family. Contains 1 GAE domain.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
References for Anti-gamma Adaptin antibody (ab21980)
This product has been referenced in:
Yi L et al. HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking. J Biol Chem285:30884-905 (2010).
Read more (PubMed: 20622010) »