Overview

  • Product nameAnti-gamma Adaptin antibody
    See all gamma Adaptin primary antibodies
  • Description
    Rabbit polyclonal to gamma Adaptin
  • Tested applicationsSuitable for: IP, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    IRLRELIRTIRTARTQAEER

    , corresponding to N terminal amino acids 4-23 of gamma Adaptin

  • Positive control
    • Rat brain lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: Whole serum
  • PurityWhole antiserum
  • Purification notes0.1ml filtered lyophilized serum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
IHC-Fr
IHC-P
  • Application notesIHC-Fr: 1/200 - 1/2000. depending on tissue and detection method.
    IHC-P: 1/200 - 1/2000. depending on tissue and detection method.
    IP: Use at an assay dependent dilution.
    WB: 1/1000 - 1/4000. depending on detection method. Predicted molecular weight: 91 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the adaptor complexes large subunit family.
      Contains 1 GAE domain.
    • Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adapter-related protein complex 1 subunit gamma-1 antibody
      • Adaptor protein complex AP 1 gamma 1 subunit antibody
      • Adaptor protein complex AP 1 subunit gamma 1 antibody
      • Adaptor protein complex AP-1 subunit gamma-1 antibody
      • Adaptor related protein complex 1 gamma 1 subunit antibody
      • ADTG antibody
      • AP 1 complex subunit gamma 1 antibody
      • AP-1 complex subunit gamma-1 antibody
      • AP1G1 antibody
      • AP1G1_HUMAN antibody
      • CLAPG1 antibody
      • Clathrin assembly protein complex 1 gamma 1 large chain antibody
      • Clathrin assembly protein complex 1 gamma large chain antibody
      • Clathrin assembly protein complex 1 gamma-1 large chain antibody
      • Clathrin associated/assembly/adaptor protein large gamma 1 antibody
      • Gamma1 adaptin antibody
      • Gamma1-adaptin antibody
      • Golgi adaptor HA1/AP1 adaptin gamma subunit antibody
      • Golgi adaptor HA1/AP1 adaptin subunit gamma 1 antibody
      • Golgi adaptor HA1/AP1 adaptin subunit gamma-1 antibody
      • MGC18255 antibody
      see all

    Anti-gamma Adaptin antibody images

    • All lanes : Anti-gamma Adaptin antibody (ab21980) at 1/2000 dilution

      Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 91 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    References for Anti-gamma Adaptin antibody (ab21980)

    This product has been referenced in:
    • Yi L  et al. HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking. J Biol Chem 285:30884-905 (2010). Read more (PubMed: 20622010) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab21980.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"