Overview

  • Product nameAnti-gamma Adaptin antibody
    See all gamma Adaptin primary antibodies
  • Description
    Goat polyclonal to gamma Adaptin
  • SpecificityRecognises a band representing AP1G1 at just below 97kD that is blocked by the immunising peptide in HeLa extract.
  • Tested applicationsWB, Electron Microscopymore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    CTAPTSKPSSAGGEL

    , corresponding to amino acids 646 - 659 of Human AP1G1.

Properties

Applications

Our Abpromise guarantee covers the use of ab3706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Electron Microscopy
  • Application notesElectron Microscopy: Use at an assay dependent dilution.
    WB: 1/500. Detects a band of approximately 94 kDa (predicted molecular weight: 91 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the adaptor complexes large subunit family.
      Contains 1 GAE domain.
    • Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adapter-related protein complex 1 subunit gamma-1 antibody
      • Adaptor protein complex AP 1 gamma 1 subunit antibody
      • Adaptor protein complex AP 1 subunit gamma 1 antibody
      • Adaptor protein complex AP-1 subunit gamma-1 antibody
      • Adaptor related protein complex 1 gamma 1 subunit antibody
      • adaptor-related protein complex 1 gamma 1 subunit antibody
      • ADTG antibody
      • AP 1 complex subunit gamma 1 antibody
      • AP-1 complex subunit gamma-1 antibody
      • AP1G1 antibody
      • AP1G1_HUMAN antibody
      • CLAPG1 antibody
      • Clathrin assembly protein complex 1 gamma 1 large chain antibody
      • Clathrin assembly protein complex 1 gamma large chain antibody
      • Clathrin assembly protein complex 1 gamma-1 large chain antibody
      • Clathrin associated/assembly/adaptor protein large gamma 1 antibody
      • clathrin-associated/assembly/adaptor protein large gamma 1 antibody
      • Gamma1 adaptin antibody
      • Gamma1-adaptin antibody
      • Golgi adaptor HA1/AP1 adaptin gamma subunit antibody
      • Golgi adaptor HA1/AP1 adaptin subunit gamma 1 antibody
      • Golgi adaptor HA1/AP1 adaptin subunit gamma-1 antibody
      • MGC18255 antibody
      see all

    Anti-gamma Adaptin antibody images



    • Predicted band size : 91 kDa


      Western blot using ab3706 at 1/500.

      Lane 1: HeLa Whole Cell Lysate
      Lane 2: HeLa Whole Cell Lysate + 1µg/ml blocking/immunising peptide

      Secondary ab: Rabbit polyclonal to Goat IgG HRP ab6741 (1/5000).
      Exposure time: 1 minute.
      Lysates at 20µg/lane.

      Predicted molecular weight: 91 kDa. The strong band just under 97kD represents AP1G1.

    References for Anti-gamma Adaptin antibody (ab3706)

    This product has been referenced in:
    • Giles DK  et al. Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion. Microbes Infect 8:1579-91 (2006). Read more (PubMed: 16698305) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"