Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma H2A.X (phospho S139) antibody [9F3]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Mouse Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous HeLa were treated with Bleomycing to induce DNA double strand breaks. They were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. The antibody produces the expected nuclear and focal staining pattern. Methanol-fixed samples were also evaluated and produced a similar strong focal staining pattern in bleomycin treated samples.
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Dr. Kirk Mcmanus

Verified customer

Submitted Feb 07 2012

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