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Other Immunogen Type corresponding to Human gamma H2A.X (phospho S140). Synthetic peptide sequence surrounding phosphorylated Ser140
Our Abpromise guarantee covers the use of ab22551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa.|
|ICC/IF||Use a concentration of 2 - 4 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Western blot analysis of Phospho-H2A.X pSer140(ab22551) at a concentration of 1 ug/ml on Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) followed by HRP conjugated goat anti-mouse lgG (H+L) Secondary Antibody.
IHC image of ab22551 staining in Human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab22551 labelling gamma H2A.X (phospho S140) in HeLa cells by immunocytochemistry/immunofluorescence.
ab22551 labelling gamma H2A.X (phospho S140) in control (left) and G2/M Arrested (right) cells by Immunocytochemistry/Immunofluorescence.
A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures.
Primary anitibody H2A.X (phospho S140) antibody [3F2], ab22551, 100ug.
Secondary antibody Mouse IgG-Fc (FITC), ab97264, 1mg.