Overview

  • Product nameAnti-gamma Sarcoglycan antibody
    See all gamma Sarcoglycan primary antibodies
  • Description
    Rabbit polyclonal to gamma Sarcoglycan
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (NP_000222.1)

  • Positive control
    • Human kidney tissue lysate, Transfected 293T cell line IHC-P: FFPE human heart tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab104478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionComponent of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • Tissue specificityExpressed in skeletal and heart muscle.
  • Involvement in diseaseDefects in SGCG are the cause of limb-girdle muscular dystrophy type 2C (LGMD2C) [MIM:253700]. LGMD2C is characterized by progressive muscle wasting from early childhood.
  • Sequence similaritiesBelongs to the sarcoglycan beta/delta/gamma/zeta family.
  • Cellular localizationCell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa dystrophin associated glycoprotein antibody
    • 35 kDa dystrophin-associated glycoprotein antibody
    • 35DAG antibody
    • 35kD dystrophin associated glycoprotein antibody
    • 35kDa dystrophin-associated glycoprotein antibody
    • A4 antibody
    • DAGA4 antibody
    • DMDA antibody
    • DMDA1 antibody
    • Gamma SG antibody
    • Gamma-sarcoglycan antibody
    • Gamma-SG antibody
    • LGMD2C antibody
    • MAM antibody
    • MGC130048 antibody
    • Sarcoglycan gamma antibody
    • SCARMD2 antibody
    • SCG3 antibody
    • SGCG antibody
    • SGCG_HUMAN antibody
    • TYPE antibody
    see all

Anti-gamma Sarcoglycan antibody images

  • IHC image of gamma Sarcoglycan staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104478, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-gamma Sarcoglycan antibody (ab104478) at 1 µg/ml + Human kidney tissue lysate at 50 µg

    Predicted band size : 32 kDa
  • All lanes : Anti-gamma Sarcoglycan antibody (ab104478) at 1 µg/ml

    Lane 1 : gamma Sarcoglycan transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 32 kDa

References for Anti-gamma Sarcoglycan antibody (ab104478)

This product has been referenced in:
  • van den Bergen JC  et al. Clinical characterisation of Becker muscular dystrophy patients predicts favourable outcome in exon-skipping therapy. J Neurol Neurosurg Psychiatry N/A:N/A (2013). Human . Read more (PubMed: 23667215) »

See 1 Publication for this product

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The sarcoglycan protein is located in the cell membrane and expressed in kidney, so the membrane kidney lyaste ab30193 should be suitable for both these sarcoglycan antibodies. However, they may like to consider a ...

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