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Synthetic peptide derived from human gamma Synuclein.
Our Abpromise guarantee covers the use of ab55424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab55424 staining gamma Synclein in Rat retina cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized by freezing and blocked with 2% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in 2% Triton + 2% Serum in PBS) for 8 hours at 4°C. An Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.