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EKEEVAEEAQSGGDcorresponds to amino acids 114-127 of the human gamma synuclein protein. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.
Our Abpromise guarantee covers the use of ab6169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab6169 was tested on recombinant protein only, 100ng recombinant protein each lane. 1= alpha-synuclein, 2 = beta-synuclein, 3 = gamma-synuclein. The primary was 1/2000, secondary was alkaline phosphatase conjugated anti-rabbit from Sigma. We did not try it on crude samples such as brain or other tissue homogenates.