The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa).
1/50 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionPlays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
Tissue specificityHighly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
Sequence similaritiesBelongs to the synuclein family.
Post-translational modificationsPhosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
Cellular localizationCytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
References for Anti-gamma Synuclein antibody [EP1539Y] (ab52633)
This product has been referenced in:
Wang Y et al. Hypoxia-inducible lncRNA-AK058003 promotes gastric cancer metastasis by targeting ?-synuclein. Neoplasia16:1094-106 (2014).
Read more (PubMed: 25499222) »
Kim SH et al. Frequent expression of follicular dendritic cell markers in Hodgkin lymphoma and anaplastic large cell lymphoma. J Clin Pathol66:589-96 (2013).
Read more (PubMed: 23454725) »