Overview

  • Product nameAnti-gamma Tubulin antibody
    See all gamma Tubulin primary antibodies
  • Description
    Rabbit polyclonal to gamma Tubulin
  • SpecificityDoes not react with alpha or beta tubulin. Immunogen sequence found in both gamma tubulin 1 and gamma tubulin 2.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Hamster, Human, African green monkey, Tribolium castaneum
    Predicted to work with: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
  • Immunogen

    Synthetic peptide:

    EEFATEGTDRKDVFFY-C

    conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin

  • Positive control
    • WB: A431, HeLa, Jurkat, C2C12, Rat2, U87, NIH3T3 and CHO cell lysates. ICC/IF: NIH3T3, HeLa and Rat2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab11317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5000.

It is recommended to fix cells with methanol/acetone.

WB 1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Anti-gamma Tubulin antibody images

  • Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

  • All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : Rat2 cell lysate
    Lane 6 : U87 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : CHO cell lysate

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

  • ab11317 staining gamma Tubulin in mouse embryonic brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in 1mM EDTA. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 20°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

References for Anti-gamma Tubulin antibody (ab11317)

This product has been referenced in:

See all 22 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Fibroblast)
Permeabilization No
Specification Fibroblast
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1%
Fixative Methanol
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Submitted Jun 22 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample African Green Monkey Cell lysate - whole cell (cos7)
Specification cos7
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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Submitted Sep 24 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Oct 14 2013

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Application Immunocytochemistry/ Immunofluorescence
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Cell (Embryonic fibroblasts)
Specification Embryonic fibroblasts
Permeabilization No
Fixative Methanol
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Submitted Jun 21 2013

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM EDTA
Sample Mouse Tissue sections (Embryonic brain)
Specification Embryonic brain
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jun 21 2013

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Application Western blot
Sample Mouse Cell lysate - whole cell (MIN6)
Loading amount 30 µg
Specification MIN6
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
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Submitted Nov 30 2012

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblast)
Specification fibroblast
Fixative Methanol
Permeabilization No
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Oct 01 2012

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (IMCD3)
Specification IMCD3
Fixative Methanol
Permeabilization No
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Oct 01 2012

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Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 60 µg
Specification Hela
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jan 11 2011

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS-7)
Loading amount 40 µg
Specification COS-7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 29 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"