Overview

  • Product nameAnti-gamma Tubulin antibody
    See all gamma Tubulin primary antibodies
  • Description
    Rabbit polyclonal to gamma Tubulin
  • SpecificityDoes not react with alpha or beta tubulin. Immunogen sequence found in both gamma tubulin 1 and gamma tubulin 2.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Hamster, Human, African Green Monkey, Tribolium castaneum
    Predicted to work with: Drosophila melanogaster, Schizosaccharomyces pombe, Sea Urchin
  • Immunogen

    Synthetic peptide:

    EEFATEGTDRKDVFFY-C

    conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin

  • Positive control
    • A431 cultured cells extract; cultured chicken fibroblasts.

Properties

Applications

Our Abpromise guarantee covers the use of ab11317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5000.

It is recommended to fix cells with methanol/acetone.

WB 1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Anti-gamma Tubulin antibody images

  • Ab11317 staining cultured hamster cells (CHO cells) by ICC. The cells were fixed with paraformaldehyde and incubated with ab11317 at a dilution of 1/5000 for 12 hours. A polyclonal goat anti-rabbit antibody conjugated to Alexa Fluor ® 488 was used as the seconday antibody.

    This image is courtesy of an Abreview submitted by Dr Jan Postberg on 7 December 2005.

    See Abreview

  • ab11317 at a 1/1000 dilution detecting gamma tubulin from human HeLa cells by ICC. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-rabbit Alexa-Fluor ® 488.

    This image is courtesy of an anonymous Abreview submitted on 7 December 2005.

    See Abreview

References for Anti-gamma Tubulin antibody (ab11317)

This product has been referenced in:
  • Van Itallie CM  et al. Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion. J Cell Sci 127:885-95 (2014). Read more (PubMed: 24338363) »
  • Wichmann C  et al. Activating c-KIT mutations confer oncogenic cooperativity and rescue RUNX1/ETO-induced DNA damage and apoptosis in human primary CD34+ hematopoietic progenitors. Leukemia N/A:N/A (2014). Read more (PubMed: 24897507) »

See all 19 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Fibroblast)
Permeabilization No
Specification Fibroblast
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1%
Fixative Methanol
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Submitted Jun 22 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample African Green Monkey Cell lysate - whole cell (cos7)
Specification cos7
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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Submitted Sep 24 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Oct 14 2013

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Application Immunocytochemistry/ Immunofluorescence
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Cell (Embryonic fibroblasts)
Specification Embryonic fibroblasts
Permeabilization No
Fixative Methanol
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Submitted Jun 21 2013

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM EDTA
Sample Mouse Tissue sections (Embryonic brain)
Specification Embryonic brain
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jun 21 2013

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Application Western blot
Sample Mouse Cell lysate - whole cell (MIN6)
Loading amount 30 µg
Specification MIN6
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
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Submitted Nov 30 2012

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblast)
Specification fibroblast
Fixative Methanol
Permeabilization No
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Oct 01 2012

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (IMCD3)
Specification IMCD3
Fixative Methanol
Permeabilization No
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Oct 01 2012

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Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 60 µg
Specification Hela
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jan 11 2011

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS-7)
Loading amount 40 µg
Specification COS-7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 29 2008

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"