The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
It is recommended to fix cells with methanol/acetone.
1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
Post-translational modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Ab11317 staining cultured hamster cells (CHO cells) by ICC. The cells were fixed with paraformaldehyde and incubated with ab11317 at a dilution of 1/5000 for 12 hours. A polyclonal goat anti-rabbit antibody conjugated to Alexa Fluor ® 488 was used as the seconday antibody.
This image is courtesy of an Abreview submitted by Dr Jan Postberg on 7 December 2005.
References for Anti-gamma Tubulin antibody (ab11317)
This product has been referenced in:
Van Itallie CM et al. Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion. J Cell Sci127:885-95 (2014).
Read more (PubMed: 24338363) »
Wichmann C et al. Activating c-KIT mutations confer oncogenic cooperativity and rescue RUNX1/ETO-induced DNA damage and apoptosis in human primary CD34+ hematopoietic progenitors. LeukemiaN/A:N/A (2014).
Read more (PubMed: 24897507) »