Overview

  • Product nameAnti-gamma Tubulin antibody
    See all gamma Tubulin primary antibodies
  • Description
    Rabbit polyclonal to gamma Tubulin
  • SpecificityThis sequence is highly specific for C. elegans gamma tubulin, and is not found in gamma tubulin sequences of other species or other members of the tubulin family such as alpha, beta, delta and epsilon tubulins.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Caenorhabditis elegans
  • Immunogen

    Synthetic peptide

    LDEYKAVVQKDYLTRGL

    with N-terminal added cysteine conjugated to KLH, corresponding to C terminal amino acids 428-444 of Caenorhabditis elegans gamma Tubulin

  • Positive control
    • Whole extract of C. elegans

Properties

Applications

Our Abpromise guarantee covers the use of ab50721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB Use a concentration of 0.75 - 1.5 µg/ml. Predicted molecular weight: 48 kDa.

Target

  • FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Alternative names
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Anti-gamma Tubulin antibody images

  • All lanes : Anti-gamma Tubulin antibody (ab50721) at 1/2000 dilution

    Lane 1 : C. elegans whole extract
    Lane 2 : C. elegans whole extract with gamma Tubulin immunizing peptide at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate
    Developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

References for Anti-gamma Tubulin antibody (ab50721)

This product has been referenced in:
  • Wiese M  et al. Regulation of neuronal APL-1 expression by cholesterol starvation. PLoS One 7:e32038 (2012). WB ; Caenorhabditis elegans . Read more (PubMed: 22363792) »
  • Wiese M  et al. Intracellular trafficking and synaptic function of APL-1 in Caenorhabditis elegans. PLoS One 5: (2010). WB ; Caenorhabditis elegans . Read more (PubMed: 20862215) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Caenorhabditis elegans Cell (embryo)
Specification embryo
Fixative Paraformaldehyde
Permeabilization Yes - freeze-cracking and methanol (5 min at -20C)
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Submitted Feb 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"