The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 48 kDa.
1/50 - 1/100.
Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
1/140. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Belongs to the tubulin family.
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling gamma Tubulin with ab180595 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Myung Park J et al. Beclin 1 and UVRAG Confer Protection from Radiation-Induced DNA Damage and Maintain Centrosome Stability in Colorectal Cancer Cells. PLoS One9:e100819 (2014).
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