Recombinant
RabMAb

Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

Overview

  • Product name
    Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker
    See all gamma Tubulin primary antibodies
  • Description
    Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Drosophila melanogaster, a wide range of other species
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human gamma Tubulin aa 1-100. The exact sequence is proprietary.
    Database link: Q9NRH3

  • Positive control
    • Hela, Jurkat, A431 or 293 lysate, Human colon tissue or Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 48 kDa.
IHC-P 1/50 - 1/100.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

Flow Cyt 1/140. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • All lanes : Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595) at 1/2000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Jurkat lysate
    Lane 3 : A431 lysate
    Lane 4 : 293 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling gamma Tubulin with ab180595 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • Flow cytometry analysis of Jurkat cells using ab180595 at a 1/140 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

References

This product has been referenced in:
  • Myung Park J  et al. Beclin 1 and UVRAG Confer Protection from Radiation-Induced DNA Damage and Maintain Centrosome Stability in Colorectal Cancer Cells. PLoS One 9:e100819 (2014). WB . Read more (PubMed: 24956373) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 26 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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