Anti-gamma Tubulin antibody [TU-30] (ab27074)

Overview

  • Product name
    Anti-gamma Tubulin antibody [TU-30]
    See all gamma Tubulin primary antibodies
  • Description
    Mouse monoclonal [TU-30] to gamma Tubulin
  • Specificity
    This antibody recognises gamma tubulin expressed on stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human, Pig, Xenopus laevis, Chinese hamster
    Predicted to work with: Plants
  • Immunogen

    Synthetic peptide:

    EYHAATRPDYISWGTQ

    conjugated to KLH, corresponding to amino acids 434-449 of gamma Tubulin.

  • Epitope
    This antibody reacts with an epitope located in the C terminal region of gamma tubulin.
  • Positive control
    • P19 cell line, 3T3 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab27074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

Target

  • Function
    Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Anti-gamma Tubulin antibody [TU-30] images

  • Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).

  • ab27074 staining gamma tubulin in human colon and thyroid tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in EDTA pH 9.0 without undergoing blocking step. The primary antibody was diluted 1/1000 and incubated with sample for 1 hour at room temperature. A HRP-conjugated goat polyclonal to mouse antibody was used undiluted, as secondary.

    See Abreview

  • ab27074 staining gamma tubulin in the Human HEK293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol (-20ºC 20 min) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 or 1/1000 in blocking solution) for 45 minutes. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC/IF. The cells were also transfected with beta-tubulin GFP which stained green.

  • Overlay histogram showing HeLa cells stained with ab27074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27074, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-gamma Tubulin antibody [TU-30] (ab27074)

This product has been referenced in:
  • Denu RA  et al. Centrosome amplification induces high grade features and is prognostic of worse outcomes in breast cancer. BMC Cancer 16:47 (2016). IHC . Read more (PubMed: 26832928) »
  • Yamada HY  et al. Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice. Carcinogenesis 36:429-40 (2015). IHC-P ; Mouse . Read more (PubMed: 25740822) »

See all 13 Publications for this product

Product Wall

Thank you for contacting us. This product has been tested for Immunohistochemistry. The reference is as follows:

Nicholas AK et al. WDR62 is associated with the spindle pole and is mutated in human microcephaly. Nat Genet 42:1010-4 (2010). IHC...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T)
Specification
HEK 293T
Fixative
Paraformaldehyde
Permeabilization
Yes - Methanol -20ºC 20 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon and Thyroid)
Specification
Colon and Thyroid
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9.0 EDTA
Username

Mr. 孝一 鎌田

Verified customer

Submitted Dec 11 2008

Thank you for your enquiry. I can tell you that this antibody has been applied in the following plant publication; Binarova P et al. Nuclear gamma-tubulin during acentriolar plant mitosis. Plant Cell 12:433-42 (2000). PubMed: 10715328 I have ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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