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Our Abpromise guarantee covers the use of ab27074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.
Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.|
Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).
ab27074 staining gamma tubulin in human colon and thyroid tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in EDTA pH 9.0 without undergoing blocking step. The primary antibody was diluted 1/1000 and incubated with sample for 1 hour at room temperature. A HRP-conjugated goat polyclonal to mouse antibody was used undiluted, as secondary.
ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC/IF. The cells were also transfected with beta-tubulin GFP which stained green.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"