Overview

  • Product nameAnti-GAMT antibody
    See all GAMT primary antibodies
  • Description
    Rabbit polyclonal to GAMT
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Pig
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

  • Tissue specificityExpressed in liver.
  • PathwayAmine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 2/2.
  • Involvement in diseaseDefects in GAMT are the cause of guanidinoacetate methyltransferase deficiency (GAMT deficiency) [MIM:612736]. GAMT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, intractable seizures and movement disturbances, severe depletion of creatine/phosphocreatine in the brain, and accumulation of guanidinoacetic acid (GAA) in brain and body fluids.
  • Sequence similaritiesBelongs to the RMT2 methyltransferase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCDS2 antibody
    • Epididymis secretory protein Li 20 antibody
    • GAMT antibody
    • GAMT_HUMAN antibody
    • Guanidinoacetate N methyltransferase antibody
    • Guanidinoacetate N-methyltransferase antibody
    • HEL-S-20 antibody
    • PIG2 antibody
    • TP53I2 antibody
    see all

Anti-GAMT antibody images

  • Anti-GAMT antibody (ab60129) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab60129 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GAMT antibody (ab60129)

ab60129 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (liver and diaphragm muscle)
Gel Running Conditions Reduced Denaturing (separation gel 10%, concentration gel 4%)
Loading amount 40 µg
Treatment intermittent hypoxia, FIO2 : 21%-6%, cycle 30s-30s, 8h/day during 35 days
Specification liver and diaphragm muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Apr 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"