Anti-Ganglioside GD3 antibody [4F6] (ab6217)

Overview

  • Product name
    Anti-Ganglioside GD3 antibody [4F6]
    See all Ganglioside GD3 primary antibodies
  • Description
    Mouse monoclonal [4F6] to Ganglioside GD3
  • Specificity
    The antibody reacts specifically with GD3 ganglioside (NeuAcá2-8NeuAcá2-3Galâ1-4Glcâ1-1Cer) and cross reacts with 9-O-AcGD3 ganglioside (9OAcNeuAcá2-8NeuAcá2-3Galâ1-4Glcâ1-1Cer) and GT1a ganglioside ganglioside (NeuAcá2-8NeuAcá2-3Galâ1-4GalNAcâ1-4(NeuAcá2-3)Galâ1-4Glcâ1-1Cer)
  • Tested applications
    Suitable for: WB, ELISA, Thin Layer Chromatographymore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: Tissue Culture Supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    4F6
  • Myeloma
    unknown
  • Isotype
    IgG3
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Thin Layer Chromatography
  • Application notes
    ELISA: 1/10 - 1/100.
    TLC: Use at an assay dependant dilution.
    WB: 1/10 - 1/100.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Almost all melanomas, astrocytomas, a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen. It is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in Humans via enhancement of cytotoxic and proliferative response of lymphocytes.
    • Alternative names
      • GD3 antibody

    References

    ab6217 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry. This antibody has not yet been tested for IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

    The subtype is IgG3 - I'll add this information to the datasheet.

    All the information we have for this monoclonal antibody is on the datasheet. It is supplied as a tissue culture supernatant, the concentration has not been determined, but there are suggested dilutions for ELISA and WB on the datasheet. You may also l...

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    This antibody has not been tested in immunohistochemistry, and so we cannot guarantee that the antibody will work. All the data that we have for this antibody is on the datasheet.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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