Overview

  • Product nameAnti-Ganglioside GD3 antibody [R24]
    See all Ganglioside GD3 primary antibodies
  • Description
    Mouse monoclonal [R24] to Ganglioside GD3
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    SK-MEL-28 Melanoma cell line

  • Positive control
    • Human melanoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.01M PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesAffinity purified from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone numberR24
  • IsotypeIgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration. PubMed: 19765188ab91537-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/40.

Target

  • RelevanceAlmost all melanomas, astrocytomas, a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen. It is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in Humans via enhancement of cytotoxic and proliferative response of lymphocytes.
  • Alternative names
    • GD3 antibody

Anti-Ganglioside GD3 antibody [R24] images

  • IHC image of ab11779 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11779, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab11779 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11779, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Ganglioside GD3 antibody [R24] (ab11779)

This product has been referenced in:
  • Tringali C  et al. Molecular subtyping of metastatic melanoma based on cell ganglioside metabolism profiles. BMC Cancer 14:560 (2014). ICC/IF ; Human . Read more (PubMed: 25085576) »
  • Ribeiro-Resende VT  et al. Mice lacking GD3 synthase display morphological abnormalities in the sciatic nerve and neuronal disturbances during peripheral nerve regeneration. PLoS One 9:e108919 (2014). IF ; Mouse . Read more (PubMed: 25330147) »

See all 6 Publications for this product

Product Wall

I am pleased to hear the customer would like to make use of our testing discount offer and test ab11779 in mouse. I have arranged the following code:

DISCOUNT CODE: #####
Expiration date: ######

This code will give you 1 free...

Read More

Thank you for your enquiry. The concentration of this antibody is 400 ug/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"