Overview

  • Product name
    Anti-Ganglioside GM1 antibody
    See all Ganglioside GM1 primary antibodies
  • Description
    Rabbit polyclonal to Ganglioside GM1
  • Specificity
    No cross-reaction with other carbohydrate epitopes except some with asialo - GM1 which is natural background in the rabbit.
  • Tested applications
    Suitable for: Thin Layer Chromatography, ICC/IF, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: African green monkey
  • Immunogen

    Purified ganglioside GM1 and complete Freunds adjuvant (Bovine).

Properties

Applications

Our Abpromise guarantee covers the use of ab23943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Thin Layer Chromatography 1/1000. Phosphate buffered saline (pH 7.4) is the recommended diluent.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ELISA 1/10240. Optimal ELISA detection ca. 50 ng of antigen.

Target

  • Relevance
    A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
  • Cellular localization
    Plasma membrane
  • Alternative names
    • GM1 ganglioside antibody
    • Monosialoanglioside antibody

Anti-Ganglioside GM1 antibody images

  • ab23943 staining Ganglioside GM1 in African Green Monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde, permeabilised in 100% methanol and then blocked using 5% serum for 1 hour at room temperature. Samples were then incubated with primary antibody at 1/10 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution. Counterstained with DAPI (1 ug/ml).

    See Abreview

  • ab23943 used in Flow Cytometry.
    CV1 cells (positive control) and GM95 cells (negative control) were washed in PBS, trypsinized, washed and incubated with ab23943 at a 1/50 dilution (black histograms) or isotpe control (grey histograms). The secondary antibody used was a DyLight 488 conjugated donkey anti-rabbit, used at a 1/200 dilution.

    CV1 cells were positively stained while GM95 showed no staining.

    See Abreview

References for Anti-Ganglioside GM1 antibody (ab23943)

This product has been referenced in:
  • Rubovitch V  et al. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury. Sci Rep 7:41269 (2017). Read more (PubMed: 28112258) »
  • LaRocca TJ  et al. Human-specific bacterial pore-forming toxins induce programmed necrosis in erythrocytes. MBio 5:N/A (2014). Read more (PubMed: 25161188) »

See all 3 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast)
Permeabilization
No
Specification
Mouse Embryonic Fibroblast
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 18 2015

The reason that species is not mentioned on the datasheet is because the target is species independent. The target is Ganglioside GM1, a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with sialic acids linked on the sugar chain...

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Thank you for your enquiry.

I can confirm that ab23943 antibody is sold as whole antiserumt. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the data...

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Thank you very much for your reply I am pleased to hear you are considering trying GM1 antibody ab31119 in ICC-IF.  Therefore, I am pleased to provide details of the testing discount program we can offer you: I can provide a discount off a future purch...

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Thank you for your enquiry. I can confirm these are different antibodies, which have been tested in different applications. Tested applications covered by our guarnatee are listed on teh online datasheets: ab23943  Anti-Ganglioside GM1 antibody...

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Application
Flow Cytometry
Sample
African Green Monkey Cell (cell culture)
Specification
cell culture
Fixation
no fixation
Permeabilization
No
Username

Mr. Nir Drayman

Verified customer

Submitted Aug 02 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

Submitted Mar 23 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (CV1 cells)
Specification
CV1 cells
Fixative
4% PFA followed by 100% Methanol
Permeabilization
Yes - 100% Methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Nir Drayman

Verified customer

Submitted Jan 04 2011

Thank you for your enquiry. I found your enquiry most interesting. Whilst the literature that you detail provides evidence that NK cells in the mouse liver can be detected using an anti-asialo GM1 antibody the two antibodies that you detail are yet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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