Anti-Ganglioside GM1 antibody (ab31119)

Overview

  • Product nameAnti-Ganglioside GM1 antibody
    See all Ganglioside GM1 primary antibodies
  • Description
    Rabbit polyclonal to Ganglioside GM1
  • SpecificityRecognizes ganglioside GM1. Expresses some reactivity to asialo GM1 due to the natural background in rabbit. Does not crossreact with other carbohydrate epitopes.
  • Tested applicationsSuitable for: ICC/IF, ELISA, Thin Layer Chromatographymore details
  • Immunogen

    Other Immunogen Type corresponding to Cow Ganglioside GM1. Immunogen=Purified monosialoganglioside GM1 and complete Freund’s adjuvant.

  • General notesAddition of glycerol as a cryopreservative is not recommended.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA 1/10000.
Thin Layer Chromatography 1/1000.

Target

  • RelevanceA member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
  • Cellular localizationPlasma membrane
  • Alternative names
    • GM1 ganglioside antibody
    • Monosialoanglioside antibody

References for Anti-Ganglioside GM1 antibody (ab31119)

ab31119 has not yet been referenced specifically in any publications.

Product Wall

The reason that species is not mentioned on the datasheet is because the target is species independent. The target is Ganglioside GM1, a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with sialic acids linked on the sugar chain...

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Thank you very much for your reply I am pleased to hear you are considering trying GM1 antibody ab31119 in ICC-IF.  Therefore, I am pleased to provide details of the testing discount program we can offer you: I can provide a discount off a future purch...

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Thank you for your enquiry. I can confirm these are different antibodies, which have been tested in different applications. Tested applications covered by our guarnatee are listed on teh online datasheets: ab23943  Anti-Ganglioside GM1 antibody...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Bronchial epithelial cells)
Specification Bronchial epithelial cells
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"