Anti-Ganglioside GM1 antibody (ab31119)

Overview

  • Product name
    Anti-Ganglioside GM1 antibody
    See all Ganglioside GM1 primary antibodies
  • Description
    Rabbit polyclonal to Ganglioside GM1
  • Specificity
    Recognizes ganglioside GM1. Expresses some reactivity to asialo GM1 due to the natural background in rabbit. Does not crossreact with other carbohydrate epitopes.
  • Tested applications
    Suitable for: ICC/IF, ELISA, Thin Layer Chromatographymore details
  • Immunogen

    Other Immunogen Type corresponding to Cow Ganglioside GM1. Immunogen=Purified monosialoganglioside GM1 and complete Freund’s adjuvant.

  • General notes
    Addition of glycerol as a cryopreservative is not recommended.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA 1/10000.
Thin Layer Chromatography 1/1000.

Target

  • Relevance
    A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
  • Cellular localization
    Plasma membrane
  • Alternative names
    • GM1 ganglioside antibody
    • Monosialoanglioside antibody

References for Anti-Ganglioside GM1 antibody (ab31119)

ab31119 has not yet been referenced specifically in any publications.

Product Wall

The reason that species is not mentioned on the datasheet is because the target is species independent. The target is Ganglioside GM1, a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with sialic acids linked on the sugar chain...

Read More

Thank you very much for your reply I am pleased to hear you are considering trying GM1 antibody ab31119 in ICC-IF.  Therefore, I am pleased to provide details of the testing discount program we can offer you: I can provide a discount off a future purch...

Read More

Thank you for your enquiry. I can confirm these are different antibodies, which have been tested in different applications. Tested applications covered by our guarnatee are listed on teh online datasheets: ab23943  Anti-Ganglioside GM1 antibody...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Bronchial epithelial cells)
Specification
Bronchial epithelial cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up