Overview

  • Product nameAnti-GAP43 antibody
    See all GAP43 primary antibodies
  • Description
    Rabbit polyclonal to GAP43
  • SpecificityThis antibody recognises the protein designated Gap43.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human, Monkey
  • Immunogen

    Purified GAP43 from cat brain.

  • General notesThis product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 25249946
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 43-57 kDa (predicted molecular weight: 28.6 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionThis protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
  • Sequence similaritiesBelongs to the neuromodulin family.
    Contains 1 IQ domain.
  • Post-translational
    modifications
    Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
  • Cellular localizationCell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Axonal membrane protein GAP 43 antibody
    • Axonal membrane protein GAP-43 antibody
    • B 50 antibody
    • Calmodulin binding protein P 57 antibody
    • F1 antibody
    • GAP 43 antibody
    • GAP43 antibody
    • Growth Associated Protein 43 antibody
    • Growth-associated protein 43 antibody
    • Nerve Growth Related Peptide antibody
    • Nerve growth related peptide GAP43 antibody
    • NEUM_HUMAN antibody
    • Neural phosphoprotein B 50 antibody
    • Neural phosphoprotein B-50 antibody
    • Neuromodulin antibody
    • Neuron growth associated protein 43 antibody
    • PP46 antibody
    • Protein F1 antibody
    • QtrA-11580 antibody
    • QtrA-13071 antibody
    see all

References for Anti-GAP43 antibody (ab11136)

This product has been referenced in:
  • Benitez SU  et al. Synaptic plasticity and sensory-motor improvement following fibrin sealant dorsal root reimplantation and mononuclear cell therapy. Front Neuroanat 8:96 (2014). IHC-FoFr ; Rat . Read more (PubMed: 25249946) »
  • Cheng C  et al. Loss of innervation and axon plasticity accompanies impaired diabetic wound healing. PLoS One 8:e75877 (2013). IHC ; Mouse . Read more (PubMed: 24098736) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (hippocampus)
Loading amount 10 µg
Specification hippocampus
Gel Running Conditions Non-reduced Denaturing (10%SDS gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Apr 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"