Overview

  • Product name
    Anti-GAP43 antibody [EP928Y]
    See all GAP43 primary antibodies
  • Description
    Rabbit monoclonal [EP928Y] to GAP43
  • Specificity
    ab75843 detects both phosphorylated and nonphosphorylated GAP43.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding serine 41 of human GAP43

  • Positive control
    • Human brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
    • Sequence similarities
      Belongs to the neuromodulin family.
      Contains 1 IQ domain.
    • Post-translational
      modifications
      Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
    • Cellular localization
      Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Axonal membrane protein GAP 43 antibody
      • Axonal membrane protein GAP-43 antibody
      • B 50 antibody
      • Calmodulin binding protein P 57 antibody
      • F1 antibody
      • GAP 43 antibody
      • GAP43 antibody
      • Growth Associated Protein 43 antibody
      • Growth-associated protein 43 antibody
      • Nerve Growth Related Peptide antibody
      • Nerve growth related peptide GAP43 antibody
      • NEUM_HUMAN antibody
      • Neural phosphoprotein B 50 antibody
      • Neural phosphoprotein B-50 antibody
      • Neuromodulin antibody
      • Neuron growth associated protein 43 antibody
      • PP46 antibody
      • Protein F1 antibody
      • QtrA-11580 antibody
      • QtrA-13071 antibody
      see all

    Images

    • Anti-GAP43 antibody [EP928Y] (ab75843) at 1/100000 dilution + human brain lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Zhang G  et al. Combined application of Rho-ROCKII and GSK-3ß inhibitors exerts an improved protective effect on axonal regeneration in rats with spinal cord injury. Mol Med Rep 14:5180-5188 (2016). Read more (PubMed: 27840930) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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