Anti-GAP43 antibody - Neuronal Marker (ab12274)

Overview

  • Product name
    Anti-GAP43 antibody - Neuronal Marker
    See all GAP43 primary antibodies
  • Description
    Rabbit polyclonal to GAP43 - Neuronal Marker
  • Tested applications
    Suitable for: IHC-P, WB, ICC, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human, Fish, Monkey
  • Immunogen

    GAP43 purified from cat brain.

  • Positive control
    • ICC/IF: SKNSH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab12274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/2000. Detects a band of approximately 43-57 kDa (predicted molecular weight: 24 kDa).
ICC 1/100 - 1/400.
IP Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/400.

Target

  • Function
    This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
  • Sequence similarities
    Belongs to the neuromodulin family.
    Contains 1 IQ domain.
  • Post-translational
    modifications
    Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
  • Cellular localization
    Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Axonal membrane protein GAP 43 antibody
    • Axonal membrane protein GAP-43 antibody
    • B 50 antibody
    • Calmodulin binding protein P 57 antibody
    • F1 antibody
    • GAP 43 antibody
    • GAP43 antibody
    • Growth Associated Protein 43 antibody
    • Growth-associated protein 43 antibody
    • Nerve Growth Related Peptide antibody
    • Nerve growth related peptide GAP43 antibody
    • NEUM_HUMAN antibody
    • Neural phosphoprotein B 50 antibody
    • Neural phosphoprotein B-50 antibody
    • Neuromodulin antibody
    • Neuron growth associated protein 43 antibody
    • PP46 antibody
    • Protein F1 antibody
    • QtrA-11580 antibody
    • QtrA-13071 antibody
    see all

Images

  • ICC/IF image of ab12274 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12274, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM



  • Predicted band size : 24 kDa

    This image is courtesy of an anonymous Abreview

    Western blot showing detection of GAP43 in Mouse Whole brain tissue lysates using ab12274 (1/5000) in conjunction with a goat anti-rabbit secondary antibody conjugated to HRP (1/10000). Ab12274 did not detect GAP43 in Sciatic nerve lysates (negative control). Western blot showing detection of GAP43 in Mouse Whole brain tissue lysates using ab12274 (1/5000) in conjunction with a goat anti-rabbit secondary antibody conjugated to HRP (1/10000). Ab12274 did not detect GAP43 in Sciatic nerve lysates (negative control).

    See Abreview

  • Ab12274 staining human neuropil. Staining is localized to the cytoplasm and membrane.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:

See all 8 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihren netten Anruf gestern und die Publikation, die Sie mir geschickt haben.

Wie versprochen, habe ich mir die Abbildung 1 angesehen und daraufhin unsere GAP43- Antikörper danach durchsucht, welche für Gefrierschnitte und Par...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (Spinal cord (on sections of whole tadpole))
Permeabilization
Yes - 0.2% Triton-X/100
Specification
Spinal cord (on sections of whole tadpole)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
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Submitted Jan 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
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Submitted Aug 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 5°C
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Submitted Nov 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
10 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (12% SDS)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, Sciatic Nerve)
Loading amount
20 µg
Specification
Brain, Sciatic Nerve
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
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Submitted Jul 04 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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