Anti-GAP43 antibody - Neuronal Marker (ab16053)
- Datasheet
- Specific References (16)
- Protocols
Overview
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Product nameAnti-GAP43 antibody - Neuronal Marker
See all GAP43 primary antibodies -
DescriptionRabbit polyclonal to GAP43 - Neuronal Marker
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SpecificityThis antibody does not perform well in IHC-P (abreview 21090, 21089)
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Tested applicationsSuitable for: ICC/IF, IHC (PFA fixed), WB, IHC-Frmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human, Chimpanzee
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat GAP43.
(Peptide available as ab16379.)
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Positive control
- This antibody gave a positive signal in Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation as well as Mouse spinal cord tissue lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab16053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. | |
| IHC (PFA fixed) | 1/300 - 1/1000. PMID-28197545 |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa).Can be blocked with Rat GAP43 peptide (ab16379). | |
| IHC-Fr | 1/500. |
Target
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FunctionThis protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
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Sequence similaritiesBelongs to the neuromodulin family.
Contains 1 IQ domain. -
Post-translational
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. -
Cellular localizationCell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
- Information by UniProt
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Database links
- Entrez Gene: 460599 Chimpanzee
- Entrez Gene: 2596 Human
- Entrez Gene: 14432 Mouse
- Entrez Gene: 29423 Rat
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- SwissProt: P06837 Mouse
see all -
Alternative names
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
see all
Anti-GAP43 antibody - Neuronal Marker images
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Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody - Neuronal Marker (ab16053)This image is courtesy of an anonymous abreview.ab16053 staining GAP43 in murine dorsal root ganglia by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol, permeabilized using 0.1% saponin/PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab16053 at a 1/500 dilution for 14 hours at 4°C. The secondary antibody used was a DyLight 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution. -
Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml
Lane 1 : spinal cord chung rat 1
Lane 2 : spinal cord chung rat 2
Lane 3 : spinal cord chung rat 3
Lysates/proteins at 20 µg per lane.
Secondary
Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size : 43-53 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 90 kDa (possible multimer). -
Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United KingdomImmunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
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Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43-53 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Protocols
References for Anti-GAP43 antibody - Neuronal Marker (ab16053)
This product has been referenced in:
- Poulsen ET et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci 10:59 (2017). ICC/IF . Read more (PubMed: 28360834) »
- Williams PA et al. Nicotinamide and WLD(S) Act Together to Prevent Neurodegeneration in Glaucoma. Front Neurosci 11:232 (2017). IHC-P ; Mouse . Read more (PubMed: 28487632) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"