Anti-GAP43 antibody - Neuronal Marker (ab16053)

Abreviews (11)Q&A (3)Specific References (19)

Overview

  • Product name
    Anti-GAP43 antibody - Neuronal Marker
    See all GAP43 primary antibodies
  • Description
    Rabbit polyclonal to GAP43 - Neuronal Marker
  • Host species
    Rabbit
  • Specificity
    This antibody does not perform well in IHC-P (abreview 21090, 21089)
  • Tested applications
    Suitable for: ICC/IF, IHC (PFA fixed), WB, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat GAP43.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab16379.)

  • Positive control
    • This antibody gave a positive signal in Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation as well as Mouse spinal cord tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IHC (PFA fixed) 1/300 - 1/1000.

PMID-28197545
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa).Can be blocked with Rat GAP43 peptide (ab16379).
IHC-Fr 1/500.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
    • Sequence similarities
      Belongs to the neuromodulin family.
      Contains 1 IQ domain.
    • Post-translational
      modifications
      Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
    • Cellular localization
      Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Axonal membrane protein GAP 43 antibody
      • Axonal membrane protein GAP-43 antibody
      • B 50 antibody
      • Calmodulin binding protein P 57 antibody
      • F1 antibody
      • GAP 43 antibody
      • GAP43 antibody
      • Growth Associated Protein 43 antibody
      • Growth-associated protein 43 antibody
      • Nerve Growth Related Peptide antibody
      • Nerve growth related peptide GAP43 antibody
      • NEUM_HUMAN antibody
      • Neural phosphoprotein B 50 antibody
      • Neural phosphoprotein B-50 antibody
      • Neuromodulin antibody
      • Neuron growth associated protein 43 antibody
      • PP46 antibody
      • Protein F1 antibody
      • QtrA-11580 antibody
      • QtrA-13071 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody - Neuronal Marker (ab16053)
      Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody - Neuronal Marker (ab16053)This image is courtesy of an anonymous abreview.
      ab16053 staining GAP43 in murine dorsal root ganglia by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed with methanol, permeabilized using 0.1% saponin/PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab16053 at a 1/500 dilution for 14 hours at 4°C. The secondary antibody used was a DyLight 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

      See Abreview

    • Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
      Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
      All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml

      Lane 1 : spinal cord chung rat 1
      Lane 2 : spinal cord chung rat 2
      Lane 3 : spinal cord chung rat 3

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution

      Performed under reducing conditions.

      Predicted band size: 43-53 kDa
      Observed band size: 43 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 90 kDa (possible multimer)

    • Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053)
      Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom

      Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).

      Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.

    • Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
      Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
      Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43-53 kDa
      Observed band size: 43 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes

    References

    This product has been referenced in:
    • Stark DT  et al. Optic Nerve Regeneration After Crush Remodels the Injury Site: Molecular Insights From Imaging Mass Spectrometry. Invest Ophthalmol Vis Sci 59:212-222 (2018). Read more (PubMed: 29340649) »
    • van der Merwe Y  et al. An Elastomeric Polymer Matrix, PEUU-Tac, Delivers Bioactive Tacrolimus Transdurally to the CNS in Rat. EBioMedicine 26:47-59 (2017). IHC-P ; Rat . Read more (PubMed: 29208469) »

    See all 19 Publications for this product

    Publishing research using ab16053? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    14 reviews or Q&A

    10
    Votes: Highest First

    We are planing to use anti-GAP43 to label regenerating axons of the sciatic nerves in mice (immunofluorescence). Ab7462 is known to work well, but it is either discontinuous or  change to another Catalog number (?). We sincerely hope you can find a rep...

    Read More

    We would recommend using ab16053 in place of the discontinued item. Ab16053 has been tested in and is guaranteed in IF on mouse samples. This product has also been featured in 5 publications which we are aware of and has excellent Abreviews. More infor...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse brain (hippocampus))
    Specification
    mouse brain (hippocampus)
    Fixative
    4% PFA fixed
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0,1% Saponin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Nov 19 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-GAP43 antibody - Neuronal Marker

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse lung (bronchus))
    Specification
    mouse lung (bronchus)
    Fixative
    Methanol
    Permeabilization
    Yes - 0,1% Saponin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Western blot abreview for Anti-GAP43 antibody - Neuronal Marker

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain tissue)
    Loading amount
    10 µg
    Specification
    brain tissue
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gel)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Nov 09 2012

    The following is the answer to your questionnaire.

    1) Abcam product code ab16053

    2) Abcam order reference number or product batch number

    Lot: GR42948-1

    3) Description of the problem

    I perform the i...

    Read More

    Thank you for your reply.

    Your protocol looks good. The one recommendation I could make would be to increase the dilution of the primary and secondary (especially) to see if that improves the staining.
    You can try the primary at 1:250 a...

    Read More

    Phone call reporting high background with ab166053 when used for immunohistochemical staining of rat brain.

    Thank you for calling us and for alerting us to the problem you are experiencing with anti-GAP43 antibody (ab16053). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As we...

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GAP43 antibody - Neuronal Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (dorsal root ganglia)
    Specification
    dorsal root ganglia
    Fixative
    Methanol
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (in situ spinal cord)
    Specification
    in situ spinal cord
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 02 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker

     Below Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Head)
    Specification
    Head
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 02 2010

    Western blot abreview for Anti-GAP43 antibody - Neuronal Marker

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (NG108-15 neuroblastoma x glioma (mouse/rat) hybrid)
    Loading amount
    10 µg
    Specification
    NG108-15 neuroblastoma x glioma (mouse/rat) hybrid
    Treatment
    DMEM+HAT, with 5% FBS for 20 hrs
    Gel Running Conditions
    Reduced Denaturing (10% acrylamide)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    abreview image
    Username

    Dr. Alan Howe

    Verified customer

    Submitted Sep 23 2009

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