Overview

  • Product name
  • Description
    Rabbit polyclonal to GAPDH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Baboon
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Turkey, Pig, Duck
  • Immunogen

    Synthetic peptide:

    DAPMFVMGVNHFKYA

    , corresponding to amino acids 126-140 of Human GAPDH.

  • Positive control
    • Rat heart protein extract. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MCF7

Applications

Our Abpromise guarantee covers the use of ab22555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 36 kDa.

Target

Images

  • Lane 1 : Anti-GAPDH antibody (ab22555)
    Lanes 2-4 : Anti-GAPDH antibody (ab22555) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 25 µg per lane.
  • ab22555 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22555 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Bavley CC  et al. Cav1.2 channels mediate persistent chronic stress-induced behavioral deficits that are associated with prefrontal cortex activation of the p25/Cdk5-glucocorticoid receptor pathway. Neurobiol Stress 7:27-37 (2017). WB ; Mouse . Read more (PubMed: 28289693) »
  • Zhou Y  et al. Asperlin Inhibits LPS-Evoked Foam Cell Formation and Prevents Atherosclerosis in ApoE-/- Mice. Mar Drugs 15:N/A (2017). WB ; Mouse . Read more (PubMed: 29135917) »

See all 16 Publications for this product

Customer reviews and Q&As

Thank you very much for your interest in anti-GAPDH antibodies.

I have reviewed the products in our catalog in effort to try an find for you a product which was most similar to ab36840. I believe that the following two products offer the bes...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Baboon Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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