Overview

  • Product nameAnti-GAPDH antibody [GA1R]See all GAPDH primary antibodies ...
  • Description
    Mouse monoclonal [GA1R] to GAPDH
  • Specificityab125247 recognizes native and denatured forms of GAPDH.
  • Tested applicationsWB, Dot Blot, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Other
    Predicted to work with: a wide range of other species
  • Immunogen

    Recombinant GAPDH.

  • Positive control
    • Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
  • General notes

    Store at 4°C (add 0.05% NaN3) for several days to weeks.

Properties

Applications

Our Abpromise guarantee covers the use of ab125247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
Dot Blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

Anti-GAPDH antibody [GA1R] images

  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate
    Lane 5 : Chicken tissue lysate
    Lane 6 : Hamster tissue lysate

    Lysates/proteins at 5 µg per lane.

    developed using the ECL technique

    Predicted band size : 36 kDa
  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : BL21 bacterial lysate
    Lane 2 : Sf9 insect lysate
    Lane 3 : Saccharomyces cerevisiae lysate

    Lysates/proteins at 5 µg per lane.

    developed using the ECL technique

    Predicted band size : 36 kDa

References for Anti-GAPDH antibody [GA1R] (ab125247)

This product has been referenced in:
  • Freisinger T  et al. Establishment of a robust single axis of cell polarity by coupling multiple positive feedback loops. Nat Commun 4:1807 (2013). WB ; Saccharomyces cerevisiae . Read more (PubMed: 23651995) »
  • Oliveras-Salvá M  et al. rAAV2/7 vector-mediated overexpression of alpha-synuclein in mouse substantia nigra induces protein aggregation and progressive dose-dependent neurodegeneration. Mol Neurodegener 8:44 (2013). WB ; Mouse . Read more (PubMed: 24267638) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 3.75e+006 cells
Gel Running Conditions Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
Sample Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
Specification whole cell extract
Blocking step 0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2013

Thank you for that feedback. I am pleased to hear that the replacement antibody has proved successful for you.

I do not completely understand why at a dilution of 1/500 you would only be able to perform two western blots. There should be enoug...

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I am very sorry for the delay in getting back to you in regards to the Western blotting protocol used with the Anti-GAPDH antibody [GA1R] ab125247.

I can now confirm that the following conditions were used to obtain the image presented on the ...

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Thank you very much for contacting Abcam.

To our knowledge, ab125247 has not been tested in Fly/Drosophila tissue. Therefore, I can offer you our AbTrial program where you can now use our products in an untested application or species without ...

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Thank you for your reply.

I would be concerned that you are getting a colour change in your Bradford reagent without any sample present. This would suggest that there is a contamination in your buffers. I would make new sterile ones to make s...

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Thank you for your reply.

I think it is very important to check how much protein is being loaded to keep it within a manageable range, loading too much can lead to aggregation of proteins and high background whilst loading too little can obvi...

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Thank you for confirming that information.

It would be helpful to have the order number if possible. I have found one order of the 17th of July delivered to the University of Copenhagen, Faculty of Health Sciences. Is this your order?
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Thank you for sharing this information.

I am currently consulting with the source of this antibody to try to understand why the results have not been as expected with this antibody.

In order to investigate the situation further, cou...

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Thank you for contacting us. I am very glad to hear that you have taken advantage of our 100% Abreview program.

I have found the review which you have submitted and wish to thank you for this. In order to take advantage of the review code,...

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Thank you for your response. It was my pleasure to help and I am glad that the replacement has worked for you.

Please let us know if there is anything else that we at Abcam might do to help you to meet your research gols.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"