Overview

  • Product name
    Anti-GAPDH antibody [GA1R]
    See all GAPDH primary antibodies
  • Description
    Mouse monoclonal [GA1R] to GAPDH
  • Host species
    Mouse
  • Specificity
    ab125247 recognizes native and denatured forms of GAPDH.
  • Tested applications
    Suitable for: WB, Dot blot, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Other
    Predicted to work with: a wide range of other species
  • Immunogen

    Recombinant GAPDH.

  • Positive control
    • Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
  • General notes

    Store at 4°C (add 0.05% NaN3) for several days to weeks.

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab125247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate
    Lane 5 : Chicken tissue lysate
    Lane 6 : Hamster tissue lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : BL21 bacterial lysate
    Lane 2 : Sf9 insect lysate
    Lane 3 : Saccharomyces cerevisiae lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa

References

This product has been referenced in:
  • Yu KE  et al. Mass Spectrometry-Based Screening Platform Reveals Orco Interactome in Drosophila melanogaster. Mol Cells 41:150-159 (2018). Read more (PubMed: 29429152) »
  • Ye Y  et al. microRNA-605 promotes cell proliferation, migration and invasion in non-small cell lung cancer by directly targeting LATS2. Exp Ther Med 14:867-873 (2017). Read more (PubMed: 28673012) »

See all 21 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
THP-1, A549, HEK293, CEMx-174
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Oct 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
3.75e+006 cells
Gel Running Conditions
Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
Specification
whole cell extract
Blocking step
0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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